We narrowed to 10,183 results for: yeast

1. pPMVAd3

   Plasmid

   #98298

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
   

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPEY-GAL-Kan

   Plasmid

   #232099

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPEY-GAL-Hyg

   Plasmid

   #232098

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPEY-Z3-Nat

   Plasmid

   #232100

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBJ1806 pRS413-COF1RFP

   Plasmid

   #37101

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSUMO-YHRC

   Plasmid

   #54336

   Purpose

   Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

   Depositor

   Claes Andréasson

   Article

   Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

   Type

   Empty backbone

   Tags

   6His-SUMO (yeast gene: SMT3)

   Expression

   Bacterial

   Promoter

   T7-LacO

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS426-GFP-Osh2-PH

    Plasmid

    #71373

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCTcon2-5INS_EV3

    Plasmid

    #102293

    Purpose

    Yeast Surface Display of 5Ins_16_EV3

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Evolved Insulin binding scFV
    

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS406-PHO5-GFP-OSBP PH domain

    Plasmid

    #58840

    Purpose

    Expresses GFP-Tagged OSBP PH domain in yeast

    Depositor

    Sean Munro, Tim Levine

    Article

    Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

    Insert

    Oxysterol-binding Protein PH domain (OSBP Human)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pG23A

    Plasmid

    #102884

    Purpose

    Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS426-GFP-Osh1-PH

    Plasmid

    #71372

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh1-PH
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa267-388

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pHO_pTEFmut7_CggR_R250A_ble

    Plasmid

    #124585

    Purpose

    Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    cggR_R250A (cggR Synthetic)
    

    Use

    Synthetic Biology

    Mutation

    changed arginine 250 to alanine

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge