We narrowed to 1,086 results for: gibson assembly

1. pCA_o-2

   Plasmid

   #202092

   Purpose

   Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MSCV Puro-pGK:GFP

   Plasmid

   #68486

   Purpose

   Retrovirus expressing GFP driven by pGK promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR and pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STAGR_gRNAScaffold_h7SK

   Plasmid

   #102842

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_h7SKpromoter
   

   Use

   As pcr template

   Promoter

   h7SK

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV Puro-CCSP:GFP

   Plasmid

   #68487

   Purpose

   Retrovirus expressing GFP driven by CCSP promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CCSP and LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MSCV Puro-CMV:GFP

   Plasmid

   #68485

   Purpose

   Retrovirus expressing GFP driven by CMV promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV and LTR

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Promoter

   hU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA_e-1

   Plasmid

   #202096

   Purpose

   Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCA_o-1

   Plasmid

   #202091

   Purpose

   Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pHAGE-UBC-MCP-mNeonGreen

    Plasmid

    #207581

    Purpose

    The lentiviral plasmids for expression of MCP-NeonGreen

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    MS2 coat protein mNeonGreen fusion
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Promoter

    UBC

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TOPO Cre-2A-GFP

    Plasmid

    #68450

    Purpose

    TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cre-2A-GFP
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA_e-2

    Plasmid

    #202097

    Purpose

    Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE:CloverCP-EFS:ZeoR

    Plasmid

    #68478

    Purpose

    Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    CloverCP
    Zeocin Resistance
    

    Use

    Lentiviral

    Tags

    "CP" degradation domain

    Expression

    Mammalian

    Promoter

    EFS and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE:shRNA A-pGK:rtTA-Cre

    Plasmid

    #68471

    Purpose

    Inducible lentivirus expressing an arbitrary shRNA and Cre

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    GFP-shRNA A-miR30
    rtTA-2A-Cre
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    pGK and pTRE

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV Puro-UBC:GFP

    Plasmid

    #68482

    Purpose

    Retrovirus expressing GFP driven by UBC promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR and UBC

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STAGR_SAMScaffold_mU6

    Plasmid

    #102845

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_mU6
    

    Use

    Pcr template for stagr inserts

    Promoter

    mU6

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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