We narrowed to 1,029 results for: gibson assembly

1. pCA_e-2

   Plasmid

   #202097

   Purpose

   Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTRE:iRFP670-EFS:Cre-2A-GFP

   Plasmid

   #68477

   Purpose

   Inducible lentivirus expressing iRFP670, Cre, and GFP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   iRFP670
   Cre-2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO Cre-2A-GFP

   Plasmid

   #68450

   Purpose

   TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Cre-2A-GFP
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE:CloverCP-EFS:ZeoR

   Plasmid

   #68478

   Purpose

   Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   CloverCP
   Zeocin Resistance
   

   Use

   Lentiviral

   Tags

   "CP" degradation domain

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV Puro-pGK:GFP

   Plasmid

   #68486

   Purpose

   Retrovirus expressing GFP driven by pGK promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO pTRE

   Plasmid

   #68457

   Purpose

   TOPO construct expressing pTRE for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Tetracycline Response Element Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE:shRNA A-pGK:rtTA-Cre

   Plasmid

   #68471

   Purpose

   Inducible lentivirus expressing an arbitrary shRNA and Cre

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   GFP-shRNA A-miR30
   rtTA-2A-Cre
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAGR_gRNAScaffold_h7SK

   Plasmid

   #102842

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_h7SKpromoter
   

   Use

   As pcr template

   Tags

   Expression

   Mutation

   Promoter

   h7SK

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV Puro-UBC:GFP

   Plasmid

   #68482

   Purpose

   Retrovirus expressing GFP driven by UBC promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and UBC

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. STAGR_SAMScaffold_mU6

    Plasmid

    #102845

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPO EFS

    Plasmid

    #68451

    Purpose

    TOPO construct expressing EFS for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Elongation Factor 1α Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV Puro-SV40:GFP

    Plasmid

    #68483

    Purpose

    Retrovirus expressing GFP driven by SV40 promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and SV40

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TOPO CCSP

    Plasmid

    #68453

    Purpose

    TOPO construct expressing CCSP for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Clara Cell Secretory Protein Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV Puro-EFS:GFP

    Plasmid

    #68484

    Purpose

    Retrovirus expressing GFP driven by EFS promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TOPO SV40

    Plasmid

    #68455

    Purpose

    TOPO construct expressing SV40 for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Simian Virus Early 40 Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNS1

    Plasmid

    #131769

    Purpose

    PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    FUSN_Fusion Red_Cry2WT
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PADH1

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. STAGR_SAMScaffold_hH1

    Plasmid

    #102847

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_hH1
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    H7SK

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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