Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 637 results for: pFAST

Showing: 561 - 580 of 637 results

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pFB1.HMBP.PrS.EZH2

Plasmid

#125161

Purpose

Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.RBBP4

Plasmid

#125164

Purpose

Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-CG-LYCHOS WT-HA-GFP

Plasmid

#199687

Purpose

Expression of LYCHOS WT in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

10xHis, EGFP, HA, HRV 3C, and His

Expression

Insect

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFBD-Btk(PH)Akt1(KD)

Plasmid

#86591

Purpose

Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)

Tags

10xHis

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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IMPT-6213

Plasmid

#99333

Purpose

AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

Depositor

Raymond Stevens

Article

Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

Insert

AGTR2 (AGTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFB-CG-LYCHOS FP>IA-HA-GFP

Plasmid

#199688

Purpose

Expression of LYCHOS FP>IA mutant in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

10xHis, 6xHis, EGFP, HA, and HRV 3C

Expression

Insect

Mutation

F43 and P44 mutated to I and A respectively

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_RFWD3:425-774

Plasmid

#210884

Purpose

Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RFWD3:S425-E774 (RFWD3 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SEC31A:1-338

Plasmid

#210925

Purpose

Insect expression of human SEC31A fragment M1 to T338. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SEC31A:M1-T338 (SEC31A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_DDA1:1-102

Plasmid

#210902

Purpose

Insect expression of full-length human DDA1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDA1:M1-T102 (DDA1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_RACK1:1-317

Plasmid

#210889

Purpose

Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_SMU1:1-513

Plasmid

#210877

Purpose

Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:M1-P513 (SMU1 Human)

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_MAGEA3:104-314

Plasmid

#210941

Purpose

Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:E104-E314 (MAGEA3 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP15:1-370

Plasmid

#210937

Purpose

Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP15:M1-H370 (UTP15 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TBL2:70-405

Plasmid

#210936

Purpose

Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TBL2:E70-H405 (TBL2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR19:1-636

Plasmid

#210933

Purpose

Insect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR19:M1-G636 (WDR19 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR73:1-378

Plasmid

#210932

Purpose

Insect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR73:M1-R378 (WDR73 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR54:1-334

Plasmid

#210931

Purpose

Insect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR54:M1-V334 (WDR54 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Showing: 561 - 580 of 637 results

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