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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: pFAST

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Showing: 561 - 580 of 856 results

  6. pCoofy41

    Plasmid
    #55184
    Purpose
    Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available Since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55184-plasmid-map-sequence-id-464534

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  9. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207087-plasmid-map-sequence-id-431238

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  15. GFP-CmTMC1truncated

    Plasmid
    #154908
    Purpose
    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-CmTMC1truncated
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-243 and 843-969
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154908-plasmid-map-sequence-id-310626

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  16. GFP-MuTMC2truncated

    Plasmid
    #154909
    Purpose
    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-MuTMC2truncated
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-179 and 691-716
    Available Since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154909-plasmid-map-sequence-id-306870

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  17. pCoofy64

    Plasmid
    #122016
    Purpose
    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Available Since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122016-plasmid-map-sequence-id-243594

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  18. pCoofy63

    Plasmid
    #122015
    Purpose
    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Available Since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122015-plasmid-map-sequence-id-243586

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  19. LHP1049 - pFB-YCK2

    Plasmid
    #32433
    Depositor
    Linda Hicke
    Insert
    YCK2 (YCK2 Budding Yeast)
    Expression
    Insect
    Mutation
    Y193L
    Promoter
    P-P10 and P-Pol
    Available Since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 580 of 856 results