We narrowed to 9,662 results for: try

1. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Tags

   Expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Promoter

   Available since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG327

   Plasmid

   #128797

   Purpose

   Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl(s)

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2R3z-BASI-ccdB-BSAI

   Plasmid

   #118389

   Purpose

   Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   ccdB
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   See Depositor Comments Section

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122090

   Purpose

   U6 driven SaCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-EF1a/b-actin

   Plasmid

   #82583

   Purpose

   5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ132-tRNA2.0

   Plasmid

   #158394

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Without promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW454 - pUFO

   Plasmid

   #115978

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Jan Lohmann

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pUFO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW103ParsR-Amp30C

    Plasmid

    #78638

    Purpose

    pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    30hrpR-30hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW101ParsR-gfp

    Plasmid

    #78636

    Purpose

    pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J101-rbs32-arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pET-28a (+)-Gal4-vp16

    Plasmid

    #171075

    Purpose

    Expresses Gal4-vp16 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and VP16 AD (413-490)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-smyhc1

    Plasmid

    #126748

    Purpose

    Zebrafish slow myosin heavy chain promoter. Clone 667

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    smyhc1 (smyhc1 Zebrafish)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E_unc503

    Plasmid

    #64020

    Purpose

    5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-NICD1

    Plasmid

    #46048

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Entry vector

    Tags

    Myc tag

    Expression

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-NR2F2

    Plasmid

    #138359

    Purpose

    Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ133-tRNA2.0

    Plasmid

    #158395

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Without promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge