We narrowed to 10,274 results for: nls
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Plasmid#130214PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyAvailable SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
TagsmCherryExpressionMammalianPromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-030 NLSNoLS-mVenus//BsaI
Plasmid#130211PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-mVenus
UseSynthetic BiologyAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-020 NLSNoLS-Neptune//BsaI
Plasmid#130201PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Neptune
UseSynthetic BiologyAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-018 NLSNoLS-Eos//BsaI
Plasmid#130199PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP
Plasmid#99729Purposeoverexpression of Tmeff2 and CopGFPDepositorAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVf-EnhCB-lacZnls mir-122 1xBS
Plasmid#35643DepositorInsert1 miR-122 target site
UseAAVPromoterCBAvailable SinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#106435PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210736PurposeCoding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Merry sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA1_allW_D262V-FLAG-IRES-eGFP
Plasmid#234621PurposeMammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.DepositorInserthnRNPA1 allW D262V (HNRNPA1 Human)
TagsFLAGExpressionMammalianMutationFamillial ALS mutation D262V, all aromatic amino …PromoterCMVAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise
Plasmid#225693PurposeLentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLSDepositorInsertrTetR-DNMT3B catalytic domain (DNMT3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGH1110
Plasmid#223682PurposeU6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastRDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC0_4_VPR
Plasmid#163716PurposePlasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertVPR
UseSynthetic Biology; Moclo vector level 0 (type 4a)Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W-Luc
Plasmid#133362PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
TagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W
Plasmid#133361PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
TagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)
Plasmid#221385PurposeLentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance geneDepositorInsertSpCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP
Plasmid#204723PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
ExpressionMammalianAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA
Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
ExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only