We narrowed to 15,187 results for: pET

1. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Constitutive (derepressed P-lac)

   Available Since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-mGreenLantern

   Plasmid

   #161912

   Purpose

   mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CapChR2_pmScarlet-N1

   Plasmid

   #188032

   Purpose

   Expresses CapChR2-mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-mGreenLantern

   Plasmid

   #161913

   Purpose

   mGreenLantern fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   araB

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-hfYFP

   Plasmid

   #191201

   Purpose

   Constitutive CAG-driven expression of hfYFP in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CAG

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLX_307 GFP

   Plasmid

   #184492

   Purpose

   Lentivirus expression for GFP

   Depositor

   Todd Golub, Peter Tsvetkov

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHL 1032

   Plasmid

   #52997

   Purpose

   pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   ompC
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp and mCherry

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon a…

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRK5 FLAG metap2

   Plasmid

   #32004

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   metap2 (Metap2 )
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pGEM-HE-h_bPAC_cmyc

   Plasmid

   #28134

   Purpose

   optogenetic tool for light modulation of cellular cAMP

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   humanized photoactivated adenylyl cyclase
   

   Use

   In vitro transcription

   Tags

   Myc

   Available Since

   June 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-HA-NBP1 (P#1261)

    Plasmid

    #14586

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pRFP-NLS

    Plasmid

    #105296

    Purpose

    To visualize nuclear dynamics in living cells

    Depositor

    Kenneth Yamada

    Article

    Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    aa encoding 172-192

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBH-UAS-secA5-YFP

    Plasmid

    #32359

    Purpose

    Live imaging of apoptotic cells in zebrafish

    Depositor

    Randall Peterson

    Article

    van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

    Insert

    secreted human annexin 5 (ANXA5 Human)
    

    Use

    Zebrafish expression

    Promoter

    UAS

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CapChR1_pmCherry-N1

    Plasmid

    #188031

    Purpose

    Expresses CapChR1-mCherry in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEYFP-N1-eKR2

    Plasmid

    #115337

    Purpose

    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

    Depositor

    Peter Hegemann

    Article

    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

    Insert

    eKR2
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pGEX NBP1 (P#415)

    Plasmid

    #14658

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
    

    Expression

    Bacterial

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pOPO717

    Plasmid

    #195302

    Purpose

    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and lacZ spacer 5 in native array
    

    Expression

    Bacterial

    Promoter

    Arabinose inducible arapBAD

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. bPAC-mycHis_pGEM

    Plasmid

    #85468

    Purpose

    Expression of photoactivated cyclase bPAC of Beggiatoa sp.

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    Bacterial Photoactivated Adenylyl Cyclase
    

    Tags

    His and Myc

    Promoter

    T7 promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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