We narrowed to 9,837 results for: pET
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Plasmid#190784PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
TagsGB1-His6-TEVExpressionBacterialMutationM165CAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
TagsGB1-His6-TEVExpressionBacterialMutationS184CAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C)
Plasmid#180027PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
TagsGB1-His6-TEVExpressionBacterialMutationS191CAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNMT2Δ191-237
Plasmid#198382PurposeBacterial expression plasmid for human DNMT2 deletion mutant; for binding assaysDepositorInsertDNMT2 (TRDMT1 Human)
TagsHis-tagExpressionBacterialMutationdeleted amino acids 191-237PromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-Tev-ALIX-V (348-702)
Plasmid#189819PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VA
Plasmid#186454PurposepTarget containing protospacer for FnCas12a targetingDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_priming_VA
Plasmid#186455PurposepTarget containing protospacer with a mismatch on position 1, for FnCas12a targetingDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VB
Plasmid#186456PurposepTarget containing protospacer for AaCas12b targetingDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.539
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
ExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (CYP8B1 Human, Staphylococcus aureus)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.544
Plasmid#184961PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
ExpressionBacterialMutationmurF donor G1359A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
ExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
ExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE5771
Plasmid#170778Purposeproduction of tagged outer membrane proteinDepositorAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE5773
Plasmid#170780Purposeproduction of tagged outer membrane proteinDepositorInsertGentR
TagspolyhisTEVSBPTEVExpressionBacterialPromoterT7Available SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP-6xHIS
Plasmid#186904PurposeBacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tagDepositorInsertsfGFP-SpyCatcher003-sfCherry1-10-6xHIS
Tags6xHis and sfGFPExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_6xH
Plasmid#185712PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged W403 to APromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only