We narrowed to 14,947 results for: pET

1. RF16

   Bacterial Strain

   #102800

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-EF1a-DIO-mGreenLantern

   Plasmid

   #164468

   Purpose

   Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   EF1a

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLX_307 FDX1

   Plasmid

   #184495

   Purpose

   Lentivirus expression for FDX1

   Depositor

   Peter Tsvetkov, Todd Golub

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   FDX1 (FDX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLM-vexGFP-Oct4

   Plasmid

   #22240

   Purpose

   2nd generation lentiviral vector

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   vexGFP_2A_OCT4 (POU5F1 synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. NA20 LN16

   Plasmid

   #101870

   Purpose

   Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

   Depositor

   Paul Clapham

   Article

   Peters et al J Virol. 2004 Jul;78(13):6915-26.

   Insert

   Env (env )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CAG-mGreenLantern

   Plasmid

   #164469

   Purpose

   Constitutive CAG-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CAG

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA-10Xcapture-PuroR

   Plasmid

   #211496

   Purpose

   Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

   Depositor

   Lesley Forrester, Antonella Fidanza

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   U6-gRNA
   Ef1a-PuroR
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 CDK9 (D167N) HA (P#811)

   Plasmid

   #14641

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant. Please see deposit…

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-TetR-neoR

   Plasmid

   #153310

   Purpose

   Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Insert

   TetR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5 FLAG DEPTOR (13×S/T->A)

    Plasmid

    #21702

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR 13xA (DEPTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable mutant. 13xA. Also has N204S …

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO mouse shRNA 1 DEPTOR

    Plasmid

    #21337

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 1 (Deptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCytERM-mGreenLantern

    Plasmid

    #164467

    Purpose

    Visualization of the ER in cells (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsNot1iso1-V1251R_R

    Plasmid

    #147376

    Purpose

    Mammalian Expression of HsNot1iso1-V1251R

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-V1251R (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO mouse shRNA 2 DEPTOR

    Plasmid

    #21338

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 2 (Deptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pLINK cyclin T1 (1-280) myc (all 200) (P#575)

    Plasmid

    #14610

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (all 200) (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    C198A, C200A, H202A, C205A. Active in 3T3 cells.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 FRT to BioID-nDIC2-3xFlag

    Plasmid

    #232418

    Purpose

    Expresses human dynein subunit DIC2 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 2 (DYNC1I2 Human)
    

    Use

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 FRT to BioID-cDIC2-3xFlag

    Plasmid

    #232416

    Purpose

    Expresses human dynein subunit DIC2 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 2 (DYNC1I2 Human)
    

    Use

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5 FRT TO GFP11-LRRK2

    Plasmid

    #231174

    Purpose

    Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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