We narrowed to 1,086 results for: gibson assembly

1. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pV2A-T

   Plasmid

   #153022

   Purpose

   Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

   Depositor

   Vito Valiante

   Article

   Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK001

   Plasmid

   #110573

   Purpose

   Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pL0_EF-H4

   Plasmid

   #202178

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pL0_EF-fcpA

   Plasmid

   #202164

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   fcpA terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pL0_AC-H4

   Plasmid

   #202122

   Purpose

   Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 promoter
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pL0_AC-P49

   Plasmid

   #202120

   Purpose

   Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   P49 promoter
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGK:HygroR-CMV:mKO2-C_UTR

   Plasmid

   #68481

   Purpose

   Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKO2-C_UTR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBTK599s

   Plasmid

   #110601

   Purpose

   BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP dropout
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Bba_R0040 TetR

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK2067

    Plasmid

    #171845

    Purpose

    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

    Depositor

    Judith Kimble

    Article

    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

    Insert

    his-58, eGFP, mCherry
    

    Tags

    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

    Expression

    Worm

    Mutation

    boxB wild type and hairpin mutant

    Promoter

    mex-5 promoter

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TOPO pGK

    Plasmid

    #68452

    Purpose

    TOPO construct expressing pGK for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Phosphoglycerate Kinase 1 Promoter (PGK1 Human)
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AP1297‐1

    Plasmid

    #87247

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    

    Tags

    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EBFP2-Kindlin2

    Plasmid

    #242822

    Purpose

    Encodes wildtype human kindlin2 and N-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

    Insert

    Kindlin2 (FERMT2 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EBFP2-K1K2

    Plasmid

    #242819

    Purpose

    Encodes human kindlin1 containing kindlin2 F3 domain and N-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

    Insert

    Kindlin1 (Fermt1 Human)
    

    Expression

    Mammalian

    Mutation

    Kindlin1 chimera containing the F3 domain of kind…

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Integrin β3-EBFP2

    Plasmid

    #242827

    Purpose

    Encodes wildtype human integrin β3 and C-terminal EBFP2 tag

    Depositor

    Cheng-han Yu

    Article

    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

    Insert

    Integrin subunit beta 3 (ITGB3 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHS1369

    Plasmid

    #239828

    Purpose

    WT OrufIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHS1623

    Plasmid

    #239833

    Purpose

    WT OrufIscB E. coli expression for recombinant purification

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB
    

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHS1376

    Plasmid

    #239829

    Purpose

    WT OrufIscB omegaRNA mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB omegaRNA scaffold
    

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge