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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,029 results for: gibson assembly

Showing: 661 - 680 of 1029 results

  1. mCherry-Dyskerin

    Plasmid
    #207577
    Purpose
    N-terminal mCherry-tag using SalI/EcoRI
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207577-plasmid-map-sequence-id-429490

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  2. pJB65

    Plasmid
    #226319
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (VTG to AAA MR1-6)-strep
    Use
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226319-plasmid-map-sequence-id-449981

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  3. pL0_EF-Fld

    Plasmid
    #202181
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    202181-plasmid-map-sequence-id-421657

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  4. pL0_EF-P49

    Plasmid
    #202176
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    P49 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202176-plasmid-map-sequence-id-410914

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  8. pL0_EF-glna

    Plasmid
    #202183
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    glna terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202183-plasmid-map-sequence-id-405699

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  9. pL0_EF-act1_tr

    Plasmid
    #202171
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    act1 truncated terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202171-plasmid-map-sequence-id-405685

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  12. pL0_EF-epyC

    Plasmid
    #202185
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202185-plasmid-map-sequence-id-405701

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  13. pL0_EF-acc2

    Plasmid
    #202184
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    acc2 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202184-plasmid-map-sequence-id-405700

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  14. pL0_EF-dph1

    Plasmid
    #202182
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    dph1 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202182-plasmid-map-sequence-id-405698

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  15. pL0_EF-NR

    Plasmid
    #202179
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    NR terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202179-plasmid-map-sequence-id-405694

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  16. pL0_EF-fcpB

    Plasmid
    #202177
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    fcpB terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202177-plasmid-map-sequence-id-405692

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  17. pL0_EF-GPI1

    Plasmid
    #202175
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GPI1 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202175-plasmid-map-sequence-id-405691

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  18. pL0_EF-vapol

    Plasmid
    #202174
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    vapol terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202174-plasmid-map-sequence-id-405690

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  19. pL0_EF-lysA

    Plasmid
    #202173
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    lysA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202173-plasmid-map-sequence-id-405688

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  20. pL0_EF-HO

    Plasmid
    #202170
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    HO terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202170-plasmid-map-sequence-id-405684

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Showing: 661 - 680 of 1029 results