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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,124 results for: gibson assembly

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Showing: 681 - 700 of 1124 results

  8. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  9. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

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  11. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87247-plasmid-map-sequence-id-173228

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  18. 3xMS-TR-100-PURO HRD

    Plasmid
    #207582
    Purpose
    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus
    Depositor
    Jens Schmidt
    Article
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    Tags
    3xMS2
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207582-plasmid-map-sequence-id-429467

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  19. pJB65

    Plasmid
    #226319
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (VTG to AAA MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226319-plasmid-map-sequence-id-449981

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Showing: 681 - 700 of 1124 results