We narrowed to 1,086 results for: gibson assembly

1. 3xMS-TR-100-PURO HRD

   Plasmid

   #207582

   Purpose

   homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
   

   Tags

   3xMS2

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB65

   Plasmid

   #226319

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis- (VTG to AAA MR1-6)-strep
   

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL0_EF-Fld

   Plasmid

   #202181

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Fld terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Feb. 2, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pL0_EF-P49

   Plasmid

   #202176

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   P49 terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Oct. 19, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pL0_DE-Per_sig_pep

   Plasmid

   #202152

   Purpose

   Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Peroxisome signal peptide
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Oct. 19, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSC-cUnaG

   Plasmid

   #207631

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL0_EF-act1_full

   Plasmid

   #202172

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   act1 full terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Sept. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pL0_EF-glna

   Plasmid

   #202183

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   glna terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pL0_EF-act1_tr

   Plasmid

   #202171

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   act1 truncated terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pL0_AC-GPI1

    Plasmid

    #202124

    Purpose

    Level 0 plasmid containing the promoter from gene 23924 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GPI1 promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pL0_AC-fcpF

    Plasmid

    #202119

    Purpose

    Level 0 plasmid containing the promoter from gene 51230 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpF promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL0_EF-epyC

    Plasmid

    #202185

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    epyC terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pL0_EF-acc2

    Plasmid

    #202184

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    acc2 terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pL0_EF-dph1

    Plasmid

    #202182

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    dph1 terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pL0_EF-NR

    Plasmid

    #202179

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    NR terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pL0_EF-fcpB

    Plasmid

    #202177

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpB terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pL0_EF-GPI1

    Plasmid

    #202175

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GPI1 terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pL0_EF-vapol

    Plasmid

    #202174

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    vapol terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pL0_EF-lysA

    Plasmid

    #202173

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    lysA terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pL0_EF-HO

    Plasmid

    #202170

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    HO terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge