We narrowed to 23,671 results for: crispr

1. pAAV-Neo_CAG-Cas9

   Plasmid

   #86698

   Purpose

   Cas9 expression vector for targeting to the AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   pCag

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-U6-sgRNA-hSyn-mCherry

   Plasmid

   #87916

   Purpose

   sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

   Depositor

   Alex Hewitt

   Article

   Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-PEmax

   Plasmid

   #187893

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Pgk

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCKO

   Plasmid

   #73311

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCGBE1-SpRY (pBM1571)

   Plasmid

   #170105

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

   Promoter

   CMV

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CARPID dCasRx-BASU

   Plasmid

   #153303

   Purpose

   express CARPID dCasRx-BASU fusion protein in mammalian cells

   Depositor

   Jian Yan, Liang Zhang

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   dCasRx-BASU
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT1 KI gRNA1

    Plasmid

    #92312

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR85

    Plasmid

    #140095

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458M-53BP1-DN1S

    Plasmid

    #131045

    Purpose

    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

    Depositor

    Punam Malik

    Article

    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

    Insert

    SpCas9-53BP1-DN1S (TP53BP1 Human)
    

    Use

    CRISPR

    Tags

    GFP and SpCas9

    Expression

    Mammalian

    Mutation

    Only expressing residues 1231-1644 of complete 53…

    Promoter

    CBH

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. JAK1 gRNA (BRDN0001145887)

    Plasmid

    #76392

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human JAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    JAK1 (JAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CDK9 gRNA (BRDN0001162576)

    Plasmid

    #77170

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK9

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK9 (CDK9 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid

    #238300

    Purpose

    Expresses nuclear version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
    

    Use

    CRISPR

    Tags

    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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