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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 9041 - 9060 of 10083 results

  1. pJK608

    Plasmid
    #71621
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to aspartate, additional N-term…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71621-plasmid-map-sequence-id-131639

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  2. pJK677

    Plasmid
    #71623
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val mutant (AaPurE1-S57V)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to valine, additional N-termina…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71623-plasmid-map-sequence-id-131640

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  3. pJK686

    Plasmid
    #71624
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asn mutant (AaPurE1-S57N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes serine-57 to asparagine, additional N-ter…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71624-plasmid-map-sequence-id-131641

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  4. pJK538

    Plasmid
    #72445
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72445-plasmid-map-sequence-id-133868

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  5. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  6. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  7. pJK620

    Plasmid
    #72537
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72537-plasmid-map-sequence-id-135983

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  8. pJK622

    Plasmid
    #72538
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72538-plasmid-map-sequence-id-132629

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  9. pJK587

    Plasmid
    #71696
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71696-plasmid-map-sequence-id-131733

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  10. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  12. PTPRJ

    Plasmid
    #38888
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRJ (PTPRJ Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C1239S inactivating mutation
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. MPP8 (3R93)

    Plasmid
    #32050
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MPP8 (MPHOSPH8 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. PP-HSP70 (3L6Q)

    Plasmid
    #25289
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:S12-N394 (cgd2_20 Cryptosporidium parvum)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S12-N394
    Available since
    Sept. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. CDY

    Plasmid
    #25507
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDY (CDY2B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    catalytic domain
    Available since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. PP-HSP70 (3L4I)

    Plasmid
    #25291
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:G15-E396
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acids G15-E396
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. ZNF289

    Plasmid
    #25232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZNF289:A3-A127 (ARFGAP2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A3-A127
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9041 - 9060 of 10083 results