We narrowed to 20,498 results for: pET

1. pRK5-HA GST RagC 120L

   Plasmid

   #19306

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding C (RRAGC Human)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Q120L

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGal4AD-CIBN

   Plasmid

   #28246

   Purpose

   Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1 (1-171aa)
   

   Use

   Tags

   Gal4AD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAF

   Plasmid

   #104152

   Purpose

   Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

   Depositor

   Robert Craigie

   Article

   Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

   Insert

   Barrier-to-autointegration factor (BAF) (BANF1 Human)
   

   Use

   Tags

   N-terminal His tag with thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Cas9-162A

   Plasmid

   #84918

   Purpose

   for in vitro transcription of Cas9 with polyA tail encoded

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Cas9
   

   Use

   Tags

   Flag, NLS, and polyA tail

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-FLPo-T2A-GFP

   Plasmid

   #161766

   Purpose

   express FLPo and GFP under the control of human synapsin promoter

   Depositor

   Baoji Xu

   Article

   An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

   Inserts

   FLPo
   T2AEGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 RagC shRNA #1

   Plasmid

   #26629

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagC shRNA (RRAGC Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. KBB418

   Plasmid

   #185088

   Purpose

   Swi6L-GFP with nuclear export signal mutated to alanines

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Swi6 nuclear export signal L to A mutations

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA GST RagA 66L

   Plasmid

   #19300

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding A (RRAGA Human)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Q66L

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-Flag-CtIP

    Plasmid

    #71108

    Purpose

    mammalian expession of Flag-CtlP

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLKO.1 RagC shRNA #2

    Plasmid

    #26630

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHyg-yeGFP-His6

    Plasmid

    #212781

    Purpose

    C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Cre

    Plasmid

    #125574

    Purpose

    Expresses Cre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-HA GST RagA 21L

    Plasmid

    #19299

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding A (RRAGA Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    T21L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-mCherry-LacR-PALB2

    Plasmid

    #71112

    Purpose

    mammalian expession of mCherry-LacR-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-PDZD8HA_sh_resistant

    Plasmid

    #105006

    Purpose

    Mammalian Expression of PDZD8, sh resistant

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    PDZD8 (Pdzd8 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The sequence recognized by shPDZD8 (TRCN000043105…

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAP003l2

    Plasmid

    #209748

    Purpose

    RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-3xFlag-ETO

    Plasmid

    #12507

    Depositor

    Dong-Er Zhang

    Article

    Biggs et al Mol Cell Biol. 2006 Oct . 26(20):7420-9.

    Insert

    ETO (RUNX1T1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-iCre

    Plasmid

    #125577

    Purpose

    Expresses iCre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge