We narrowed to 15,460 results for: pET
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Plasmid#120081PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaP)
Plasmid#83507PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta . Deletion of residues 187-283PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaC)
Plasmid#83506PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta C. Contains residues 1-339PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gLacZ.Cas9-T2A-GFP
Plasmid#170361PurposeNegative control plasmid used for Crispr/cas9 based disruption.DepositorInsertCas9-T2A-GFP
UseLentiviralPromoterU6Available SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM4889 (pCV0001)
Plasmid#120079PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (214-513aa)
Plasmid#78095Purposemammalian expression of GADD34 214-513 aaDepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationexpresses aa 214-513PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_436
Plasmid#49667DepositorInsertECF15_436
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.13.EFS-NS.H2B-RFP
Plasmid#170388PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa11_987
Plasmid#49661DepositorInsertECF11_987
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0001
Plasmid#42241PurposegRNA targeted to zebrafish gene apoeaDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
WPXL LEFTY2 gRNA
Plasmid#101924PurposeLEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.DepositorInsertLEFTY2 enhancer targeting gRNA
UseLentiviralAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mADAM8 deltaMP in pQCXIP cFLAG
Plasmid#19149DepositorAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_524
Plasmid#49668DepositorInsertECF15_524
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only