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MT2A_beta-domain

Plasmid

#200300

Purpose

Bacterial expression plasmids for production of recombinant beta-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_alpha-domain

Plasmid

#200302

Purpose

Bacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

alpha-domain of metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- BAD-YFP

Plasmid

#186567

Purpose

pMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

BAD promoter

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delM2_H

Plasmid

#146521

Purpose

Insect Expression of DmGW182-delM2

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-delM2 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1-1248_H

Plasmid

#146507

Purpose

Mammalian Expression of HsTNRC6A_1-1248

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6A_1-1248 (TNRC6A Human)

Expression

Mammalian

Mutation

254-1962 truncated version of the sequence given …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-dsRNAres_G

Plasmid

#146431

Purpose

Insect Expression of DmGW182-dsRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-dsRNAres (gw Fly)

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-del-M-GW-Cterm_F

Plasmid

#146324

Purpose

Insect Expression of DmGW182-del-M-GW-Cterm

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-del-M-GW-Cterm (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-12xmut_F

Plasmid

#146320

Purpose

Insect Expression of DmGW182-12xmut

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-12xmut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-C-term_F

Plasmid

#146294

Purpose

Mammalian Expression of HsTNRC6A-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-Cterm (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delAGOhook_F

Plasmid

#146295

Purpose

Mammalian Expression of HsTNRC6A-delAGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delAGOhook (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI_F

Plasmid

#146296

Purpose

Mammalian Expression of HsTNRC6A-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMII_F

Plasmid

#146297

Purpose

Mammalian Expression of HsTNRC6A-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMII (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-12xmut_E

Plasmid

#146251

Purpose

Insect Expression of DmGW182-12xmut

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-12xmut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC

Plasmid

#148386

Purpose

Insect Expression of DmMe31B-QHTRKKtoAAAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTRKKtoAAAAAA (me31B Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC

Plasmid

#148387

Purpose

Insect Expression of DmMe31B-R307AK313AK314A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-R307AK313AK314A (me31B Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pENmRFPCNAL2 pc1054

Plasmid

#167567

Purpose

The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

Depositor

Cristina Cardoso

Article

Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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