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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,958 results for: pET

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Showing: 9601 - 9620 of 9958 results

  11. deltaLoop-MIDAS

    Plasmid
    #171605
    Purpose
    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.
    Depositor
    Tarun Kapoor
    Publication
    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117
    Insert
    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    Tags
    His6 tag and SNAP tag
    Expression
    Bacterial
    Mutation
    Replaced amino acids 4458-4496 with ASGSGS linker
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171605-plasmid-map-sequence-id-337861

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  13. CMV-10xPP7-Qb model based

    Plasmid
    #158203
    Purpose
    Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.
    Depositor
    Roee Amit
    Publication
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158203-plasmid-map-sequence-id-311815

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  14. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Ruma Banerjee
    Publication
    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d
    Inserts
    mutA
    mutB
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    138296-plasmid-map-sequence-id-292603

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  15. 6DUB (LOC149281)

    Plasmid
    #119967
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    LOC149281 (METTL11B Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 58-278
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119967-plasmid-map-sequence-id-232691

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  16. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101880-plasmid-map-sequence-id-194708

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  17. TJP3

    Plasmid
    #25310
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TJP3:G508-L808:cH6 (TJP3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids G508-L808
    Available since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

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  19. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    T. J. Kappock
    Publication
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71571-plasmid-map-sequence-id-130928

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  20. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Publication
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

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Showing: 9601 - 9620 of 9958 results