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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 9701 - 9720 of 10083 results

  3. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  6. pmSod1-Q42L;Q123A-N’His

    Plasmid
    #117710
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L; Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117710-plasmid-map-sequence-id-227524

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  7. 5T1G (CBX1)

    Plasmid
    #101884
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX1 (CBX1 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    101884-plasmid-map-sequence-id-194720

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  8. 2QO2 (EPHA3)

    Plasmid
    #101271
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPHA3 (EPHA3 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101271-plasmid-map-sequence-id-193505

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  9. 2QNR (NEDD5)

    Plasmid
    #101273
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD5 (SEPTIN2 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101273-plasmid-map-sequence-id-193507

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  11. pJK605

    Plasmid
    #72530
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72530-plasmid-map-sequence-id-141993

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  12. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

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  13. pJK501

    Plasmid
    #72246
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Gln206 to Arg and changes tryptophan-151 to pheny…
    Promoter
    T7 and endogenous
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72246-plasmid-map-sequence-id-139355

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  15. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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  16. pJK624

    Plasmid
    #72539
    Purpose
    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72539-plasmid-map-sequence-id-132631

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  17. pJK564

    Plasmid
    #71581
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)
    Depositor
    T. J. Kappock
    Publication
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes serine-71 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71581-plasmid-map-sequence-id-130940

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  18. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    T. J. Kappock
    Publication
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71571-plasmid-map-sequence-id-130928

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Showing: 9701 - 9720 of 10083 results