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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_006009.2

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMS13

Plasmid

#229474

Purpose

Expresses RssB with Q254A & N256A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A, N256A)

Expression

Bacterial

Mutation

Q254A, N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF42

Plasmid

#229467

Purpose

Expresses RssB with S29C & E229C mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (S29C, E229C)

Expression

Bacterial

Mutation

S29C, E229C

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS26

Plasmid

#229471

Purpose

Expresses RssB with D104N mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104N)

Tags

10xHis

Expression

Bacterial

Mutation

D104N

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS23

Plasmid

#229470

Purpose

Expresses RssB with D104A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Tags

10xHis

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#229462

Purpose

Expresses IraD with Q68A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q68A)

Tags

6xHis

Expression

Bacterial

Mutation

Q68A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMS12

Plasmid

#229461

Purpose

Expresses IraD with W64R mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (W64R)

Tags

6xHis

Expression

Bacterial

Mutation

W64R

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF40

Plasmid

#229459

Purpose

Expresses IraD with R70A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R70A)

Tags

6xHis

Expression

Bacterial

Mutation

R70A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF26

Plasmid

#229458

Purpose

Expresses IraD with Q84A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q84A)

Tags

6xHis

Expression

Bacterial

Mutation

Q84A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDB179-Cut190*SS

Plasmid

#218706

Purpose

Encodes codon optimized Cut190*SSenzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

Cut190*SS

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-ICCG-I6M

Plasmid

#218690

Purpose

Encodes codon optimized LCC-ICCG I6M enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-ICCG

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-ICCG

Plasmid

#218687

Purpose

Encodes codon optimized LCC-ICCG enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-ICCG

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-SfCut

Plasmid

#218708

Purpose

Encodes codon optimized SfCut enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

SfCut

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-547 aa

Plasmid

#222657

Purpose

Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-547 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated histidine 548 to a stop codon

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-409 aa

Plasmid

#222658

Purpose

Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-409 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated proline 410 to a stop codon

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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