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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,485 results for: cas9

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Showing: 11041 - 11060 of 14485 results

  1. pJK413

    Plasmid
    #155372
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155372-plasmid-map-sequence-id-309000

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  2. pJK429

    Plasmid
    #155376
    Purpose
    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    155376-plasmid-map-sequence-id-308997

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  6. lentiCRISPR - C3orf17 sgRNA 1

    Plasmid
    #70652
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C3orf17
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid
    #70655
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. lentiCRISPR - C9orf114 sgRNA 2

    Plasmid
    #70654
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pADH118-17

    Plasmid
    #91727
    Purpose
    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91727-plasmid-map-sequence-id-180525

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  10. pADH143-2

    Plasmid
    #90990
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90990-plasmid-map-sequence-id-180523

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  12. p305N-5

    Plasmid
    #246288
    Purpose
    Evaluation of AtU6.1m2 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1m2 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    A-to-C (-63 from TSS) and G-to-T (-57) within USE
    Promoter
    AtU6.1m2 promoter
    Available Since
    Dec. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    246288-plasmid-map-sequence-id-494268

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  14. p305N-30

    Plasmid
    #246313
    Purpose
    Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    PtU6.2c3 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletion: -T (-29 from TSS) within TATA
    Promoter
    PtU6.2c3 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246313-plasmid-map-sequence-id-491198

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  18. p305N-8

    Plasmid
    #246291
    Purpose
    Evaluation of AtU6.29c14 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.29c14 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    T-to-C (-1 from TSS)
    Promoter
    AtU6.29c14 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246291-plasmid-map-sequence-id-491184

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  19. p305N-6

    Plasmid
    #246289
    Purpose
    Evaluation of AtU6.1m3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1m3 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-T (-57 from TSS) within USE
    Promoter
    AtU6.1m3 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246289-plasmid-map-sequence-id-491182

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Showing: 11041 - 11060 of 14485 results