We narrowed to 14,485 results for: cas9

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-EGFP

Plasmid

#75159

Purpose

Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-PE2-SpG-P2A-GFP

Plasmid

#159980

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpG-P2A-GFP

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRepair-MPP8_mAID-P2A-BFP

Plasmid

#175036

Purpose

Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

mAID-P2A-BFP

Use

Hdr donor template

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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ePPE-spG

Plasmid

#183096

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nspGCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpGCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

Plasmid

#196971

Purpose

Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Inserts

Cas9-RT
rtTA3G-P2A-eGFP

Expression

Mammalian

Promoter

PGK and TREG3G

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8e SpG Puro

Plasmid

#235044

Purpose

Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

ABE8e, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-mAID-RPA194

Plasmid

#247355

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with mAID

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

mAID

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-HaloTag-RPA194

Plasmid

#247353

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

HaloTag

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CBEd SpG UGI Puro

Plasmid

#235045

Purpose

Cytosine base editor d with SpG nicking Cas9 makes C > T edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

CBEd, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-HaloTag-UBF

Plasmid

#247351

Purpose

CRISPR/Cas9 donor plasmid to tag human UBF with Halo tag

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

UBF (UBTF) (UBTF Human)

Use

CRISPR

Tags

HaloTag

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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