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pcDNA3.1(-).TagRFP.T-2A-V5-T403D.Tau2N4R-WPRE

Plasmid

#226393

Purpose

Expresses tau propagation ORF with V5 tag on T403D mutant 2N4R tau

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T403D, Tau2N4R

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-T181D.Tau2N4R-WPRE

Plasmid

#226394

Purpose

Expresses tau propagation ORF with V5 tag on T181D mutant 2N4R tau

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T181D, Tau2N4R

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-T231D.Tau2N4R-WPRE

Plasmid

#226395

Purpose

Expresses tau propagation ORF with V5 tag on T231D mutant 2N4R tau

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T231D, Tau2N4R

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.20D3Q.Tau2N4R

Plasmid

#226397

Purpose

Expresses his-tagged, 3C-cleavable 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Mutation

20D3Q, Tau2N4R

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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p40651-EFSNS-FMR1_e1ΔCGG-24xms2

Plasmid

#222969

Purpose

MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence

Depositor

Xinyu Zhao

Publication

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-ΔCGG 5' UTR and exon1

Expression

Mammalian

Mutation

31 CGG repeats deleted from 5' UTR

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

Plasmid

#217012

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Publication

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7 (S164D, T170E)_del327-346 (CDK7 Human)

Tags

TEV cleavable his tag

Expression

Insect

Mutation

(S164D, T170E)_del327-346

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Nova1-GFP

Plasmid

#217033

Purpose

Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

Depositor

Chaolin Zhang

Publication

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Nova1 (Nova1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Publication

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIDAF

Plasmid

#213710

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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