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We narrowed to 1,284 results for: gibson assembly

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Showing: 1121 - 1140 of 1284 results

  1. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  2. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

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  6. pBD21_037

    Plasmid
    #109154
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    iRFP720
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109154-plasmid-map-sequence-id-209237

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  8. pBD21_033

    Plasmid
    #109150
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    Sirius
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109150-plasmid-map-sequence-id-209216

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  9. pBD21_036

    Plasmid
    #109153
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    mKO2
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109153-plasmid-map-sequence-id-209235

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  10. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

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  11. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  12. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

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  15. pBD21_035

    Plasmid
    #109152
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    EGFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    109152-plasmid-map-sequence-id-209236

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  17. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Article
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

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  19. pHR-HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

    Plasmid
    #246867
    Purpose
    Express HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag (M2-1exo-SunTag) for RSV imaging.
    Depositor
    Marvin Tanenbaum
    Article
    Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586
    Insert
    HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    The RSV M2-1 protein with a C-terminal insertion …
    Promoter
    SFFV
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    246867-plasmid-map-sequence-id-496508

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Showing: 1121 - 1140 of 1284 results