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plx313-EGFRwt

Plasmid

#257719

Purpose

Express EGFR WT in mammalian cells; Geneticin/G418 selection

Depositor

Jennifer Doudna

Publication

Zeng et al Nature. 2026 Jun 8. doi: 10.1038/s41586-

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF-1alpha

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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MiniCoopR CRISPRi 2xU6:gRNA, mitfa:dCas9-KRAB-MeCP2

Plasmid

#251742

Purpose

melanocyte-specific CRISPRi

Depositor

Megan Insco

Publication

Tanu et al Cell Rep. 2026 Jun 24;45(7):117526. doi:

Type

Empty backbone

Use

CRISPR

Available since

June 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-ARch3.0:EYFP

Plasmid

#248667

Purpose

Expresses Archaerhodopsin3 tagged to eYFP in Cre-expressing cells under the CAG promoter for neuronal inhibition.

Depositor

Gordon Fishell

Publication

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

Arch3.0-EYFP

Use

AAV

Tags

eYFP

Expression

Mammalian

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Publication

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTA-noI

Plasmid

#249819

Purpose

Control plasmid for null expression of recombinant protein when compared to pTA-M+ (see Table 1 of publication)

Depositor

Yuk-Ching Tse-Dinh

Publication

Sandhaus et al Antimicrob Agents Chemother. 2016 Jun 20

Type

Empty backbone

Expression

Bacterial

Promoter

Tet

Available since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mStayGold

Plasmid

#234549

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein

Depositor

Hajime Hirase

Publication

Knak et al Neurophotonics. 2025 Jun;12(Suppl 2):S22

Insert

Alb-mStayGold

Use

AAV

Mutation

None

Promoter

P3

Available since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-StayGold

Plasmid

#213790

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-StayGold fusion protein

Depositor

Hajime Hirase

Publication

Knak et al Neurophotonics. 2025 Jun;12(Suppl 2):S22

Insert

Alb (Alb Mouse)

Use

AAV

Tags

StayGold

Expression

Mammalian

Available since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS

Plasmid

#238279

Purpose

For overexpression of CFP-LacI-NUT-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS

Expression

Mammalian

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS-FtoG

Plasmid

#238280

Purpose

For overexpression of CFP-LacI-NUT-KS-FtoG

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS-FtoG

Expression

Mammalian

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT

Plasmid

#238278

Purpose

For overexpression of CFP-LacI-NUT

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT

Expression

Mammalian

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Publication

Chen et al Fundam Res. 2025 Jun 30;5(6):2993-3000.

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for HCT116

Plasmid

#238245

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Publication

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Publication

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-noKS

Plasmid

#238265

Purpose

For overexpression of V5-nb-noKS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-noKS

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS-FtoG

Plasmid

#238256

Purpose

For overexpression of EGFP-BRD4-NUT-KS-FtoG

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS-FtoG

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoG

Plasmid

#238292

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoG

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS-FtoG

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS

Plasmid

#238290

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Plasmid

#238286

Purpose

For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAML2-KS

Plasmid

#238276

Purpose

For overexpression of mEGFP-YAP-MAML2-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mEGFP-YAP-MAML2-KS

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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