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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

Plasmid

#162804

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI_IRES_Blasticidin

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0543

Plasmid

#133493

Purpose

Expression of nucleo-cytoplasmic mCherry in P-cells

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0624

Plasmid

#133491

Purpose

Expression of cytoplasmic 3mTagBFP2

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0750

Plasmid

#133484

Purpose

Expression in fission yeast from promoter purg1 , integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0471

Plasmid

#133490

Purpose

Expression of nucleo-cytoplasmic mTagBFP2

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-NC

Plasmid

#105123

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Publication

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mVenus and tagRFP

Expression

Plant

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-CN

Plasmid

#105120

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Publication

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mVenus and tagRFP

Expression

Plant

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB527

Plasmid

#74869

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB209

Plasmid

#68600

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 mCherry L3-L2

Plasmid

#62150

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Gateway Cloning

Expression

Mammalian

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV tdTomato R4-R3

Plasmid

#62151

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV iRFP L5-L2

Plasmid

#62157

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMuLE ENTR SV40 iRFP L3-L2

Plasmid

#62160

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Gateway Cloning

Expression

Mammalian

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP L5-L2

Plasmid

#62140

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP L1-R5

Plasmid

#62142

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV mCherry R4-R3

Plasmid

#62147

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Publication

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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JMJD1CA-c257

Plasmid

#53664

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

JMJD1CA

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only
CDC42BPAA-c004

Plasmid

#53658

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

CDC42BPAA

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only
SOCS4B-c002

Plasmid

#53669

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

SOCS4B

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only