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pGGG-Sr43

Plasmid

#186974

Purpose

Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

Depositor

Brande Wulff

Article

Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

Insert

Sr43

Tags

None

Expression

Bacterial and Plant

Mutation

None

Promoter

Natice

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NLS

Plasmid

#133023

Purpose

NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE ZO1myc

Plasmid

#30318

Depositor

Alan Fanning

Article

Fanning et al Mol Biol Cell. 2007 Mar . 18(3):721-31.

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

myc

Expression

Mammalian

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
RF2

Bacterial Strain

#62070

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE33

Plasmid

#90375

Purpose

AP1 - c-Fos, c-Jun, ATF, and JDP gene family gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AP1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr-AcGFP1-ORP9-PH

Plasmid

#214270

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-142

Plasmid

#118558

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD6

Plasmid

#195844

Purpose

Expresses human FZD6 with an N-terminal HiBiT; contains M345L

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD6 (FZD6 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

M345L

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmRE-Tn5-147

Plasmid

#118526

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F0

Plasmid

#184165

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Mfn2-HA S27A

Plasmid

#139193

Purpose

Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Tags

3HA

Expression

Mammalian

Mutation

Serine 27 to Alanine

Promoter

CMV promoter

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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KA1158_pPBCAG-MerCreMer-IZ

Plasmid

#124184

Purpose

piggyBac-based expression of the tamoxifen-inducible Cre

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

MerCreMer-IRES-Zeo

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE144

Plasmid

#118496

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-133

Plasmid

#118519

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-141

Plasmid

#118525

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE166

Plasmid

#118514

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE152

Plasmid

#118502

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE147

Plasmid

#118499

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PEP111E

Plasmid

#97397

Purpose

binary construct of plastid-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Plastid target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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