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plentiCRISPR-v2 Puro CHKA_sg2

Plasmid

#218523

Purpose

sgRNA targeting human CHKA

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v1 GFP FLVCR1_sg5

Plasmid

#218521

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lneg-INTR-GFP

Plasmid

#208902

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

Depositor

Christian Gruber

Publication

Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

Insert

INTR

Tags

EGFP

Expression

Mammalian

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIBY55

Plasmid

#213709

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Publication

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA

Plasmid

#206179

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S192A, S194A

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS

Plasmid

#206180

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA

Plasmid

#206181

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S194A

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA-R206H

Plasmid

#206182

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S194A, R206H

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA-R206H

Plasmid

#206183

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S192A, S194A, R206H

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-VSAS-R206H

Plasmid

#206185

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

T189V, S192A, R206H

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-VASS-R206H

Plasmid

#206186

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

T189V, S190A, R206H

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Expression

Mammalian

Mutation

KO of both AP1 sites

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT255-ameRS

Plasmid

#202309

Purpose

ameRS repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

ameRS

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT252-amtR

Plasmid

#202307

Purpose

amtR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

amtR

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT251-bm3R1

Plasmid

#202306

Purpose

bm3R1 repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

bm3R1

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP067-PcymRC_v2

Plasmid

#202302

Purpose

PCymRC_v2-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PCymRC_v2-YFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT243-Pcin

Plasmid

#202301

Purpose

PCin-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pcin-YFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP067-Pttg

Plasmid

#202300

Purpose

PTtg-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTtg-YFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT238-Pbad

Plasmid

#202298

Purpose

PBad-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pbad-YFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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