We narrowed to 5,091 results for: codon optimized

1. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-eSpCas9 (without sgRNA)

   Plasmid

   #92354

   Purpose

   Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Use

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH847-gst-optsc

   Plasmid

   #96908

   Purpose

   Plasmid for yeast expression of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   yeast codon optimised glutathione-S transferase
   

   Use

   Tags

   8xhistidine

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH861-gst-optec P210T

   Plasmid

   #89595

   Purpose

   Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed proline 210 to threonine

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH846-gst-dissc

    Plasmid

    #96907

    Purpose

    Plasmid for yeast expression of GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon dis-optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH860-gst-optec A180Q

    Plasmid

    #89594

    Purpose

    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed alanine 180 to glutamine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTH858-gst-optec V156E

    Plasmid

    #89592

    Purpose

    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed valine 156 to glutamic acid

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH859-gst-optec Y172M

    Plasmid

    #89593

    Purpose

    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed tyrosine 172 to methionine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-DBNF-mCherry

    Plasmid

    #187195

    Purpose

    To express the mouse BDNF tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    BDNF
    

    Use

    Lentiviral

    Tags

    fluorescent protein mCherry

    Expression

    Mammalian

    Mutation

    The codons have been optimized for better express…

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid

    #218193

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

    Depositor

    Florence Pojer

    Insert

    PNGaseF
    

    Use

    Tags

    tufA-His-CBD-3C

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-354

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid

    #169389

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP16
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. m.3047-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #211336

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3047-Left TALE-G1397-N-DddA11-mCherry
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. m.5147-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #211338

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

    Depositor

    Monkol Lek

    Insert

    m.5147-Left TALE-G1397-N-DddA11-mCherry
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-NT-3-mCherry

    Plasmid

    #190201

    Purpose

    To express the mouse NT-3 tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    NT-3
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge