We narrowed to 2,364 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFPB5A

   Plasmid

   #83556

   Purpose

   Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   GAL1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ePPEplus

   Plasmid

   #205241

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC65

   Plasmid

   #118865

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, mGFP

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-XMAS-1xStop

   Plasmid

   #164411

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pveg

   Plasmid

   #55173

   Purpose

   Very strong constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   veg promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   veg

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Promoter

   FCP and U6

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. amilCP chromoprotein promoter-less

    Plasmid

    #118008

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGPA1

    Plasmid

    #115476

    Purpose

    Generation of mariner-based transposon mutant libraries in Enterococcus faecium

    Depositor

    Willem van Schaik

    Article

    Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-TS2126 RnlA-Strep

    Plasmid

    #159350

    Purpose

    Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

    Depositor

    Takashi Ito

    Article

    Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

    Insert

    TS2126 RNA ligase
    

    Tags

    His6 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDECKO_GFP

    Plasmid

    #72619

    Purpose

    Expresses two gRNAs targeting GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward GFP
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

    Plasmid

    #89693

    Purpose

    Luciferase reporter plasmid targeted at csr-1 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gsy-1 promoter, luciferase
    

    Use

    Yeast episomal vector

    Tags

    luciferase

    Promoter

    gsy-1

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL302-HA-SUMO2

    Plasmid

    #235497

    Purpose

    Expression of HA-SUMO2

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    SUMO2 (SUMO2 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCDH-CAG-IntCCCre-T2A-CopGFP

    Plasmid

    #160501

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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