We narrowed to 3,239 results for: zebrafish
-
Plasmid#198059PurposemCerulean fused to human H2B proteinDepositorInsertH2B
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCeruleanPromotersp6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP
Plasmid#218857PurposeXla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.DepositorAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E ubb polyA
Plasmid#195977PurposeGateway p3E vectorDepositorInsertubb:polyA (ubb Zebrafish)
UseOtherAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-EGFP
Plasmid#127591PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionExpressionMammalianAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180480PurposeInducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert13x QUAS-ZsGreen-P2A
UseSynthetic BiologyAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-MCS, α-cry:mCherry
Plasmid#180481PurposeInducible gene expression vector p13xQUAS-MCS, α-cry:mCherryDepositorInsertMultiple cloning site
UseSynthetic BiologyAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQFDBD-2x AD*-VP16*, α-cry:EGFP
Plasmid#180473PurposeGene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFPDepositorInsertQFDBD-2xQFAD*-VP16*
UseSynthetic BiologyAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p9xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180479PurposeInducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert9x QUAS-ZsGreen-P2A
UseSynthetic BiologyAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p17xQUAS-Luciferase, α-cry:mCherry
Plasmid#180482PurposeInducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherryDepositorInsertsLuciferase
17x QUAS
UseSynthetic BiologyAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDI-QUAD-V5-pcDNA3.1
Plasmid#153550PurposePDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.DepositorInsertProtein disulphide isomerase
TagsV5 tagMutationC53S, C56S, C397S, C400SPromoterpCMVAvailable SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-mCerulean3
Plasmid#127592PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mCherry-Rab8A
Plasmid#194333PurposeFor use in light-induced Rab8a protein inactivation through the interaction with CRY2DepositorTagsmcherryExpressionMammalianPromoterCMV IE94Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
Tags3xFLAGExpressionMammalianPromoterCMV IE94 PromoterAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 1.5kb promoter/AcGFP
Plasmid#98104PurposeMouse aB crystallin 1.5kb promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPPromoterAlpha B-crystallin 1.5 kb promoter fragment (-150…Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
Tags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSGFPnERT(#205)
Plasmid#184065Purposecyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInserteGFP-nls-ERT2
UseSynthetic BiologyExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only