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pAT-WT-Myc

Plasmid

#177717

Purpose

ER protein alpha-1-antitrypsin (WT), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

Plasmid

#20943

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_LuxSit_i_mTagBFP2

Plasmid

#194300

Purpose

Mammalian expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Tags

mTagBFP2

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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791 p3E-2A-pCS2MCSpA v2

Plasmid

#195973

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-multiple cloning site

Use

Other

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCB24 pDEST crybb1:TagBFP

Plasmid

#195987

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCK051 p5E exorh:mCerulean

Plasmid

#195952

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 WT

Plasmid

#198201

Purpose

FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR223-LMP1

Plasmid

#195805

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP1 (LMP-1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-TWIST2

Plasmid

#197935

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-CA TAG126

Plasmid

#105836

Purpose

Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 126

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pME minprom KalTa4

Plasmid

#195964

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter KalTA4 (Gal4)

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
dCas9-10xSunTag(22aa)

Plasmid

#215745

Purpose

Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

dCas9-10xSunTag

Expression

Mammalian

Promoter

chicken beta-actin promoter

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F2

Plasmid

#24226

Purpose

Mammalian expression of human E2F2 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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