We narrowed to 5,469 results for: Gateway entry

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

Plasmid

#224500

Purpose

Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA-pA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p2R3a-VenusYFP-3AT

Plasmid

#71269

Purpose

Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway

Tags

4xGly linker and T3A pea Pisum sativum ribulose-1…

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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p2R3a-3xVenusYFP-OcsT

Plasmid

#71271

Purpose

Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

3 times VenusYFP

Use

Gateway

Tags

octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-IRES_ECFP

Plasmid

#48354

Purpose

Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

IRES_ECFP

Use

Gateway entry vector

Mutation

ECFP translation is mediated by an IRES. Contains…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-hs-miR-126 (JDW 1401)

Plasmid

#242564

Purpose

Gateway middle entry clone containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artificial intron encoding miR-126 (MIR126 Human)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

Plasmid

#242559

Purpose

Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP WPRE bGH pA (JDW 488)

Plasmid

#224540

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-WPRE-bGH-pA

Use

Gateway cloning

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls BFP FLAG WPRE bGH pA (JDW 484)

Plasmid

#224539

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-mTagBFP2-FLAG_WPRE-bGH-pA

Use

Gateway cloning

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-bGH-pA (JDW 1221)

Plasmid

#224527

Purpose

A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-bGH

Use

Gateway cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-dr_Unc (JDW 1365)

Plasmid

#224482

Purpose

Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Unc-503 promoter

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-V5-n2StayGoldC4

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5 mScarlet-I (JDW 1323)

Plasmid

#224487

Purpose

Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

myr-mTagBFP2-Flag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E EFS-rtTA/rtTA3 (JDW 1214)

Plasmid

#224533

Purpose

A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA

Use

Gateway cloning

Promoter

EFS

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

Plasmid

#224534

Purpose

A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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