We narrowed to 3,760 results for: biorxiv
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Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
Plasmid#207171Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expressionDepositorInsertGal4DBD-HIF1a-CAD Myc tag linker
UseTags2xMyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet_OPTN-thrombin-GST
Plasmid#216843PurposeFor expression in E. coli of OPTN (Internal ref. SMC1789)DepositorInsertOptineurin (OPTN Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG4 CTF-FLAG
Plasmid#217702PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG4 (ADGRG4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2721PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTF-FLAG
Plasmid#217694PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-566PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
Plasmid#217688PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD2 (ADGRD2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-636PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD1 CTF-FLAG
Plasmid#217687PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD1 (ADGRD1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-544PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
Plasmid#216404PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56, Glutamine 70, 443 and Asp…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB3 CTF-FLAG
Plasmid#217683PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB3 (BAI3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-856PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF5 CTF-FLAG
Plasmid#217698PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF5 (ADGRF5 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-990PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG1 CTF-FLAG
Plasmid#217699PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG1 (ADGRG1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-382PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPO-His-G-Ca-FLITS
Plasmid#191455PurposeBacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTagsHisExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-GCaMP3
Plasmid#191460PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent proteinDepositorInsertGCaMP3
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-K-GECO1
Plasmid#191469PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertK-GECO1
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-R-GECO1
Plasmid#191471PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertR-GECO1
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked mRuby3 VLP
Plasmid#182430PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP-mRuby3
deltaVP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed mRuby3 VLP
Plasmid#182431PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
deltaVP1
VP2C-mRuby3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only