We narrowed to 6,904 results for: hwa

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

Plasmid

#159659

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-delta6xNHL-LIN-41

Plasmid

#52722

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 593–868

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S163/164A

Plasmid

#33089

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S163A, S164A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Opto-GPR4

Plasmid

#106001

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR4 (GPR4 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beta(4)Integrin-DeltaCYT

Plasmid

#16264

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

truncated cytoplasmic tail introduced a stop cod…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pUC19-E6MBW6

Plasmid

#103102

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-CUL1-3'UTR

Plasmid

#97156

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

CUL1 3'UTR (CUL1 Human)

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-Lin28b

Plasmid

#64795

Purpose

Expresses human Lin28b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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IKK2-EYFP

Plasmid

#111196

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

IKKbeta (IKBKB Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12

Plasmid

#39516

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

Plasmid

#16752

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-trunc before DEP (DVL3 Human)

Tags

flag

Expression

Mammalian

Mutation

truncated before DEP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
RBM38

Plasmid

#155899

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM38 (RBM38 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) 5xGRE-NanoLuc

Plasmid

#207170

Purpose

GAl4 response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

Inserts

NanoLuciferase
d2eGFP
5x GRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Promoter

Gal4 response element minP and PGK

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-S61A,N64A,T65A

Plasmid

#104362

Purpose

Expresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acids: S61A,N64A,T65A

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-E3CY56

Plasmid

#103075

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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