We narrowed to 4,627 results for: pcr

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pAAV-hSyn-GRAB_NE1h

Plasmid

#123309

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE sensor GRAB_NE1h

Use

AAV

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pH4R-mCherry-N1

Plasmid

#84328

Purpose

Histamine-4 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

Plasmid

#194680

Purpose

CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Hpep1-betaArrestin2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NEmut

Plasmid

#123310

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE control sensor GRAB_NEmut

Use

AAV

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHMR1A

Plasmid

#102957

Purpose

gene cloning of MR1A

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

Insert

MR1A cDNA (MR1 Human)

Use

Cloning

Mutation

Stop codon has been removed by HindIII site

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHCD1E

Plasmid

#102956

Purpose

gene cloning of human CD1E

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

CD1E cDNA (CD1E Human)

Use

Cloning

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2h

Plasmid

#208701

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 hFZD4

Plasmid

#115908

Purpose

Expresses human FZD4 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GACh2.0

Plasmid

#121921

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor GACh2.0

Use

AAV

Promoter

hSyn

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Human, Synthetic)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAG491

Plasmid

#226747

Purpose

CRISPRi guide RNA against MTCH2 (dual guide)

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PITX3-eGFP-PGK-Puro

Plasmid

#31942

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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P119 C-Check

Plasmid

#66817

Purpose

Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

Depositor

Yonglun Luo

Article

Zhou et al Cell Mol Life Sci. 2016 Jan 11.

Insert

truncated Enhanced Green Fluorescent Protein and asRED

Expression

Mammalian

Promoter

PGK and CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_Smc4-mAID-HaloTAG

Plasmid

#217658

Purpose

Repair template to endogenously tag SMC4 with mAID-Halo Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

SMC4-mAID-HaloTag (SMC4 Human)

Expression

Bacterial and Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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