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We narrowed to 1,126 results for: gibson assembly

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Showing: 521 - 540 of 1126 results

  1. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  2. C71m

    Plasmid
    #120983
    Purpose
    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    CinR Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120983-plasmid-map-sequence-id-236042

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  3. pBP-TagR_MCD22

    Plasmid
    #204104
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD22 weak RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204104-plasmid-map-sequence-id-413905

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  4. pBP-TagR_MCD8

    Plasmid
    #204105
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD8 weak RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204105-plasmid-map-sequence-id-413906

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  5. pBP-TagR_MCD10

    Plasmid
    #204101
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD10 medium RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204101-plasmid-map-sequence-id-413902

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  6. pBP-TagR_MCD7

    Plasmid
    #204102
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD7 medium RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204102-plasmid-map-sequence-id-413903

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  7. pBP-TagR_MCD15

    Plasmid
    #204103
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD15 medium RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204103-plasmid-map-sequence-id-413904

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  8. pBP-TagR_MCD2

    Plasmid
    #204098
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD2 strong RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204098-plasmid-map-sequence-id-413899

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  9. pBP-TagR_MCD1

    Plasmid
    #204099
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD1 strong RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204099-plasmid-map-sequence-id-413900

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  10. pBP-TagR_MCD17

    Plasmid
    #204100
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD17 medium RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204100-plasmid-map-sequence-id-413901

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  11. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  12. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  13. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  14. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  15. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  16. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  17. C70m

    Plasmid
    #120982
    Purpose
    MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).
    Depositor
    Richard Murray
    Insert
    RpaR Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120982-plasmid-map-sequence-id-236041

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  18. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  19. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  20. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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Showing: 521 - 540 of 1126 results