Addgene

We narrowed to 1,014 results for: gibson assembly

Showing: 521 - 540 of 1014 results

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T2m

Plasmid

#121029

Purpose

MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file

Depositor

Richard Murray

Insert

ECK120033736 (164.6x Voigt terminator), short attachment

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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T7m

Plasmid

#121030

Purpose

MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

S. pyogenes tracrRNA terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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T13m

Plasmid

#121032

Purpose

MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

ECK120033737 (second strongest natural Voigt terminator)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C105m

Plasmid

#121008

Purpose

MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

sfYFP-AAV ssrA (has bbsI)

Use

Synthetic Biology

Tags

AAV degradation tag for ssrA

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C106m

Plasmid

#121009

Purpose

MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

sfYFP-DAS ssrA (has bbsI)

Use

Synthetic Biology

Tags

DAS degradation tag for ssrA

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C109m

Plasmid

#121011

Purpose

MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

mfLon protease with pdt3 tag

Use

Synthetic Biology

Tags

pdt3 mfLon degradation tag

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C70m

Plasmid

#120982

Purpose

MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).

Depositor

Richard Murray

Insert

RpaR Adam Meyer

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C41m

Plasmid

#120974

Purpose

MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

dCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C61m

Plasmid

#120977

Purpose

MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

RpaR from Scott and Hasty 2016

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C62m

Plasmid

#120978

Purpose

MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

TraR(W) from Scott and Hasty 2016

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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P61m

Plasmid

#120953

Purpose

MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).

Depositor

Richard Murray

Insert

Tac promoter Adam Meyer

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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P16m

Plasmid

#120940

Purpose

MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

ohAB carb pCinLacO m

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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P32m

Plasmid

#120943

Purpose

MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

PlacO (Tim Lu, Matt Bennett, etc)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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P35m

Plasmid

#120946

Purpose

MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).

Depositor

Richard Murray

Insert

pLbda-CasRQi

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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psLIB CMV

Plasmid

#229953

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB U6

Plasmid

#229956

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_25bpLinker

Plasmid

#157788

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_25bp linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_30bpLinker

Plasmid

#157789

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge