We narrowed to 6,448 results for: nls
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Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC130: pAAV.CMV-CasRx-VEGFA sgRNA
Plasmid#203443PurposePlasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNADepositorInsertsU6-VEGFA sgRNA
RfxCas13d
UseAAVTagsHA and NLSExpressionMammalianPromoterCMV and U6Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK459 (dTAG-BromoTag-mAID-EGFP)
Plasmid#214387PurposedTAG-BromoTag-mAID-EGFP reporter (PiggyBac)DepositorInsertdTAG-BromoTag-mAID-EGFP reporter
ExpressionMammalianAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB-HDAC5(mut-H-A)
Plasmid#114397PurposeFor PYL-HDAC5 H1006A expressionDepositorInsertPYL-HDAC5 H1006A
Tags3xFlag-NLS (internal)ExpressionMammalianMutationD593E compared to NCBI ref NP_005465.2PromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB-EXPR(PYL-cat_dom_HDAC5)
Plasmid#114401PurposeFor PYL-HDAC5 catalytic domain expressionDepositorInsertPYL-HDAC5 catalytic domain
Tags3xFlag-NLS (internal)ExpressionMammalianMutationS220F and the deletion of M221PromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah
Plasmid#199264PurposeSingle AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse modelDepositorInsertNmeABE8e
UseAAV and CRISPRTagsNLSExpressionMammalianAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e VPR
Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_D-MS2
Plasmid#177161PurposeExpresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cellsDepositorInsertsMCP-VP64
D-MS2
TagsSV40 NLSExpressionYeastPromoterADH1 promoter and SNR52 promoterAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_A-PP7
Plasmid#177156PurposeExpresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cellsDepositorInsertsPCP-VP64
A-PP7
TagsSV40 NLSExpressionYeastPromoterADH1 promoter and SNR52 promoterAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_C-MS2
Plasmid#177160PurposeExpresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cellsDepositorInsertsMCP-VP64
C-MS2
TagsSV40 NLSExpressionYeastPromoterADH1 promoter and SNR52 promoterAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMlCas12a
Plasmid#174678PurposeExpresses 6xHis-MBP tagged MlCas12aDepositorArticleInserthuMlCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBfCas12a
Plasmid#174672PurposeExpresses 6xHis-MBP tagged BfCas12aDepositorArticleInserthuBfCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-nsGFP
Plasmid#132975Purposegata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backboneDepositorInsertsnuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTags6x myc and SV40 NLSAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA
Plasmid#108820PurposeExpresses aa252 to C-terminus of Cre recombinase fused to pMag CIDDepositorInsertCre recombinase fragment aa252 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA
Plasmid#108822PurposeExpresses aa257 to C-terminus of Cre recombinase fused to pMag CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA
Plasmid#108800PurposeExpresses aa397 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA
Plasmid#108804PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only