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AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

Plasmid

#239596

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-YAP5SA

Plasmid

#223189

Purpose

Plasmid containing the Zfr/YAP5SA cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

YAP5SA (Yap1 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_g5-HT2h

Plasmid

#208715

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAS133

Plasmid

#211504

Purpose

AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mTagBF2

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS134

Plasmid

#211505

Purpose

AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

Venus

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201820

Purpose

An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-2pabPAC

Plasmid

#234547

Purpose

to express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Depositor

Hajime Hirase

Article

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

2pabPAC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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DRH016_ AAV-hSyn-DN-THR

Plasmid

#225089

Purpose

Expression of dominant-negative thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF0.5

Plasmid

#208660

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-PGC-1alpha-IRES-mCitrine

Plasmid

#241791

Purpose

Expression vector for mouse Pgc-1alpha with mCitrine

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

mCitrine

Promoter

hSyn

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN

Plasmid

#206967

Purpose

Expresses TadA7.10 and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA7.10, SpG N

Use

AAV

Promoter

CMV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA8e-SpG N aa2-713-InteinN

Plasmid

#206968

Purpose

Expresses TadA8e and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA8e, SpG N

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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