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pPW3758 : CMVd3-HsIRE1-HaloTag

Plasmid

#185680

Purpose

Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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STAgR_Neo

Plasmid

#102992

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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3xFLAG-Halo-Dyskerin

Plasmid

#207578

Purpose

CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

HaloTag DKC1

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2abcde

Plasmid

#80619

Purpose

pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only
pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Tags

Expression

Mutation

Promoter

U6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-PtBle

Plasmid

#202180

Purpose

Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

ShBle expression cassette

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Tags

Expression

Mutation

ARS sequence mutated to remove sapI site for dome…

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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DEP-GFP

Plasmid

#109043

Purpose

Expression of the Dishevelled DEP domain

Depositor

Mariann Bienz

Article

Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

Insert

dishevelled segment polarity protein 2 (DVL2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Dvl2 DEP domain

Promoter

CMV

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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