Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,126 results for: gibson assembly

Clear keywords
Showing: 661 - 680 of 1126 results

  3. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

    Enlarge

  9. pV2A-T

    Plasmid
    #153022
    Purpose
    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter
    Depositor
    Vito Valiante
    Article
    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    153022-plasmid-map-sequence-id-314021

    Enlarge

  10. pNS3

    Plasmid
    #131770
    Purpose
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites
    Depositor
    Jared Toettcher
    Article
    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d
    Insert
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    Use
    Integration into δ-sites
    Expression
    Yeast
    Mutation
    E490G to make Cry2 into Cry2Olig
    Promoter
    PADH1
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    131770-plasmid-map-sequence-id-257191

    Enlarge

  18. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

    Enlarge

  19. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

    Enlarge

Showing: 661 - 680 of 1126 results