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We narrowed to 1,118 results for: gibson assembly

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Showing: 681 - 700 of 1118 results

  13. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  19. pBN089

    Plasmid
    #173451
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
    Depositor
    James Naismith
    Publication
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173451-plasmid-map-sequence-id-344804

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Showing: 681 - 700 of 1118 results