We narrowed to 13,339 results for: GAN

1. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PA-mCherry-miniSOG-Paxillin-22

   Plasmid

   #57796

   Purpose

   Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Paxillin
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-H2B-Venus

   Plasmid

   #87614

   Purpose

   Venus (YFP) fused to histone H2B.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   H2B-Venus (H2BC8 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZJOM156

   Plasmid

   #133636

   Purpose

   Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   SEC7-2GFP (SEC7 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. iMAP-61

   Plasmid

   #187460

   Purpose

   gRNA array of iMAP-61

   Depositor

   Tian Chi

   Article

   Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

   Insert

   sgRNA array
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified U6

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGIP*35

   Plasmid

   #26776

   Purpose

   Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   GFP*35
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK0_003

   Plasmid

   #123925

   Purpose

   Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cas9-sgRNA destination
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PA-mCherry-miniSOG-Lysosomes-20

   Plasmid

   #57793

   Purpose

   Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   LAMP1
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   D50E in LAMP1

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSF1C-A-RFP

    Plasmid

    #172431

    Purpose

    StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSO221 Phis-72 BirA GFP

    Plasmid

    #45100

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    BirA::GFP
    unc-119 (unc-119 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    his-72 and unc-119

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZJOM25

    Plasmid

    #133672

    Purpose

    Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-MSP1-2GFP (MSP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM-AAV-CAG-EGFP

    Plasmid

    #153186

    Purpose

    CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_PDCD6_EF-hand

    Plasmid

    #110009

    Purpose

    Protein expression and purification of PDCD6_EF-hand

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PDCD6_EF-hand (PDCD6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK0_051

    Plasmid

    #123948

    Purpose

    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hAAVS1 landing pad destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

    Plasmid

    #32479

    Purpose

    Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAML141F-GlyR-IRES-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNA-CARtcr-IRESpuro

    Plasmid

    #89346

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 49-189

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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