We narrowed to 13,668 results for: GAN

1. pET21-pelB-GST-2

   Plasmid

   #228984

   Purpose

   For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-2
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMKO.1 puro hTERT shRNA

   Plasmid

   #10688

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   hTERT shRNA (TERT Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-EF1a-Ngn3ER-PuroR-mCherry

   Plasmid

   #197385

   Purpose

   To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   Ngn3ER-2A-PuroR-2A-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDIRECT_25F

   Plasmid

   #91143

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

   Plasmid

   #87694

   Purpose

   Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Cre-PhoCl-ERT2
   

   Expression

   Mammalian

   Promoter

   Chicken beta actin

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-RAB-AKARev

   Plasmid

   #118478

   Purpose

   ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-AKARev
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-loxP-mTagBFP2-stop-pA-loxP

   Plasmid

   #75158

   Purpose

   Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   loxP-mTagBFP2-stop-pA-loxP
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-ER-AKAR4

   Plasmid

   #64733

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   AKAR4
   

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

    Plasmid

    #233538

    Purpose

    A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-85b

    Plasmid

    #53282

    Purpose

    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, crtY, crtI
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-Flavidin

    Plasmid

    #89881

    Purpose

    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K80R, K121R, K132R, K134R

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SUPV3L1-HA WT

    Plasmid

    #232348

    Purpose

    Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    SUPV3L1 (SUPV3L1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge