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pEMS2031

Plasmid

#49134

Purpose

AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple305-iCre

Use

AAV

Expression

Mammalian

Promoter

OLIG1

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pNTI826

Plasmid

#216634

Purpose

cpTurboID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-ts-citrine-ER

Plasmid

#192575

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2118

Plasmid

#49143

Purpose

AAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Mirjalili Mohanna et al Gene Ther. 2023 Apr 19. doi: 10.1038/s41

Insert

ssAAV-smCBA-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

smCBA

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

Plasmid

#207862

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q, N496F)

Plasmid

#170139

Purpose

AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-ADAR2-DD(E488Q, N496F)

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-ARL13B-BioID2-BioID2-HA

Plasmid

#253927

Purpose

Expresses ARL13B-BioID2 in astrocytes

Depositor

Baljit Khakh

Insert

ARL13B-BioID2-BioID2-HA (ARL13B Aquifex aeolicus, Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

gfaABC1D

Available Since

July 21, 2026

Availability

Academic Institutions and Nonprofits only

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pGEP116[EF1a-mNeonGreen]-pAAVS1-SA-Neo-TRE3Gs-NGN2-2A-Zeo-CAG-TETON3G

Plasmid

#243805

Purpose

Integrates dox-inducible hNGN2 into the human AASV1 safe harbor locus for directed differentiation of neural cells from stem cells

Depositor

Michael Wells

Insert

NGN2 (NEUROG2 Human)

Use

TALEN

Promoter

TRE3GS

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-αCD3-scFv-1-SpyCatcher

Plasmid

#258659

Purpose

Mammalian expression vector encoding SpyCatcher-linked αCD3-scFv-1

Depositor

Prashant Mali

Article

Graham et al ACS Synth Biol. 2026 May 23. doi: 10.102

Insert

αCD3-scFv-1-SpyCatcher

Expression

Mammalian

Available Since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-190

Plasmid

#99664

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain ( 125-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65(150-261)-RTA(125-190)

Plasmid

#99674

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-P65(151-261)-RTA(125-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

Plasmid

#207859

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AV0.1-BWS-copGFP

Plasmid

#124967

Purpose

Express copGFP under EF1 promoter

Depositor

Kazuhiro Oka

Insert

copGFP

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CAR

Plasmid

#171964

Purpose

Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS-iBiT-Lg-MCS-BSD

Plasmid

#245343

Purpose

CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19

Depositor

Lienhard Schmitz

Article

Dreute et al Plasmid. 2026 May 23;135:102772. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Large BiT

Expression

Mammalian

Available Since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-iBiT-MCS-Lg-BSD

Plasmid

#245344

Purpose

CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19

Depositor

Lienhard Schmitz

Article

Dreute et al Plasmid. 2026 May 23;135:102772. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

LgBiT

Expression

Mammalian

Available Since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-iBiT-MCS-Sm-Puro

Plasmid

#245345

Purpose

CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19

Depositor

Lienhard Schmitz

Article

Dreute et al Plasmid. 2026 May 23;135:102772. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Small BiT

Expression

Mammalian

Available Since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-iBiT-Sm-MCS-Puro

Plasmid

#245346

Purpose

CRISPR/Cas9 mediated integration of the iBiT-system (doxycycline inducible NanoBiT) into the AAVS1 safe-harbor locus on chromosome 19

Depositor

Lienhard Schmitz

Article

Dreute et al Plasmid. 2026 May 23;135:102772. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Small BiT

Expression

Mammalian

Available Since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_Khalf-Halo

Plasmid

#247485

Purpose

Expresses H1.2 (Khalf) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Half of Lysine in C-terminal IDR are replaced by …

Promoter

Tet-responsive element

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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