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TS-PE2-C-WPRE

Plasmid

#176650

Purpose

Expresses C-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

C-terminus of PE2
Artificial SA
WPRE
bGH poly(A)

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

None

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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TS-PE2-N

Plasmid

#176649

Purpose

Expresses N-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

N-terminus of PE2
Artificial SD

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV and None

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Rat, Aquifex aeolicus)

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

Plasmid

#190898

Purpose

Entry vector for multiple sgRNA cloning

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2044

Plasmid

#79652

Purpose

ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple255-EmGFP WPRE

Use

AAV

Promoter

PAX6 HS234Z Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2116

Plasmid

#49141

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

ssAAV-Ple155-emGFP

Use

AAV

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2049

Plasmid

#79657

Purpose

ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple260-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2045

Plasmid

#79653

Purpose

ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple256-EmGFP WPRE

Use

AAV

Promoter

PAX6 enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Promoter

PAX6 MiniPromoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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DNMT3ACD-CRY2-EGFP

Plasmid

#82556

Purpose

Encodes DMNT3 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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TET1CD-CRY2-EGFP

Plasmid

#82555

Purpose

Encodes TET1 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

TET1 (TET1 Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2043

Plasmid

#79651

Purpose

ssAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple254-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVp_eGFP

Plasmid

#241002

Purpose

For targeted gene knock-in at NHP IGH locus and constitutive expression of GFP

Depositor

Jennifer Adair

Article

Castelli et al Mol Ther. 2026 Feb 2:S1525-0016(26)00081

Insert

eGFP (eGFP Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pVH4p_eGFP

Plasmid

#241003

Purpose

For targeted gene knock-in at NHP IGH locus and B cell-specific expression of GFP

Depositor

Jennifer Adair

Article

Castelli et al Mol Ther. 2026 Feb 2:S1525-0016(26)00081

Insert

eGFP (eGFP )

Use

AAV and CRISPR

Expression

Mammalian

Promoter

VH4

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CRY2-STIM1(238-685)

Plasmid

#248293

Purpose

An expression construct encoding a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685) (STIM1 )

Tags

FLAG

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-STIM1(238-685)

Plasmid

#248297

Purpose

An expression construct encoding an EGFP-fused STIM1 fragment (a.a.238-685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP-fused STIM1 fragment (a.a.238-685) (STIM1 )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-CIBN-STIM1(238-685)

Plasmid

#248296

Purpose

An expression construct encoding a CIBN-fused STIM1 fragment (a.a.238-685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CIBN-fused STIM1 fragment (a.a.238-685) (STIM1 Human)

Tags

CIBN

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-CRY2-STIM1(318-450)

Plasmid

#248294

Purpose

An expression construct encoding a CRY2-fused truncated STIM1 fragment (a.a.318–450).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-317,451-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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