We narrowed to 13,668 results for: GAN

1. pTC394

   Plasmid

   #91224

   Purpose

   protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

   Plasmid

   #47387

   Purpose

   allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   glutamate-gated chloride Channels
   

   Expression

   Mammalian

   Mutation

   Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-5xTetO-pEF-H2B-Citrine-SV40

   Plasmid

   #186966

   Purpose

   Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   5xTetO-pEF-H2B-Citrine-SV40
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   5xTetO-pEF-1alpha

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTC392

   Plasmid

   #91223

   Purpose

   protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBbsr2-5102HRasCT(Picchu)

   Plasmid

   #209917

   Purpose

   A FRET biosensor for tyrosine kinase activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   5102HRasCT
   

   Tags

   YPet and nTurquoise-GL

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE60 R196M wgMDH

   Plasmid

   #204273

   Purpose

   Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R196M

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE60 D193E wgMDH

   Plasmid

   #204269

   Purpose

   Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D193E

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-EF1-IRES-NEO mutant ITR

   Plasmid

   #67905

   Purpose

   PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM11

   Plasmid

   #133637

   Purpose

   Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX Vav1 Y3F uGFP

    Plasmid

    #14558

    Depositor

    Joan Brugge

    Article

    Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

    Insert

    Vav1 (VAV1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y3F (Y142, Y160, Y174) in DH domain

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

11. SG3ΔENV CA E28A,E29A

    Plasmid

    #145785

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residues Glutamic Acid residues 28…

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Expression

    Worm

    Promoter

    Pmex-5

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZJOM1

    Plasmid

    #133632

    Purpose

    Integration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-GFP-SED5 (SED5 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQTEV-PSMD10

    Plasmid

    #31332

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

    Plasmid

    #37604

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP II C843A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C843A

    Promoter

    CMVt

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEGIP*35

    Plasmid

    #26776

    Purpose

    Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP*35
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid

    #118407

    Purpose

    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-NES
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

    Plasmid

    #32479

    Purpose

    Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAML141F-GlyR-IRES-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Promoter

    Synapsin

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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