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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAT-mWasabi-G

Plasmid

#164170

Purpose

Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

tetO

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Promoter

Ple341

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Expression

Mammalian

Promoter

TNNT1-B

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2050

Plasmid

#49135

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CaM-NanoLuc-15aa-uTEVp

Plasmid

#163034

Purpose

FLiCRE protease with luciferin-gating for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-NanoLuc-15aa-uTEVp

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

Plasmid

#194249

Purpose

Expresses 3 non-targeting miRNAs

Depositor

Michael J Fox Foundation MJFF

Insert

miRNegative

Use

AAV and RNAi

Promoter

CAG

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CRY2-STIM1(238-685)

Plasmid

#248293

Purpose

An expression construct encoding a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685) (STIM1 )

Tags

FLAG

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-CRY2-STIM1(318-450)

Plasmid

#248294

Purpose

An expression construct encoding a CRY2-fused truncated STIM1 fragment (a.a.318–450).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-317,451-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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JD135

Plasmid

#229491

Purpose

Ef1a promoter driving expression of adenoviral 100k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

100k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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JD181

Plasmid

#229495

Purpose

Ef1a promoter driving expression of adenoviral 23k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

23k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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JD73

Plasmid

#229482

Purpose

Adenoviral VA RNA I & II genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA I & II

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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JD182

Plasmid

#229488

Purpose

Ef1a promoter driving expression of adenoviral L4-22k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

L4-22k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD81

Plasmid

#229481

Purpose

Adenoviral VA RNA II gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA II

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD183

Plasmid

#229489

Purpose

Ef1a promoter driving expression of adenoviral L4-33k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

L4-33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD92

Plasmid

#229478

Purpose

CMV promoter driving expression of adenoviral E4orf6 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4orf6

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-CaM-uTEVp

Plasmid

#163028

Purpose

FLiCRE protease for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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FRB_LD 0_CS G_tTA-BFP

Plasmid

#58876

Purpose

MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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