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MKC89_CCR5(UbC-tEPOR-2A-YFP)

Plasmid

#232404

Purpose

AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

Depositor

Kyle Cromer, Matthew Porteus

Article

Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-delVP1-VP1-588TAG

Plasmid

#240123

Purpose

Express AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al Mol Ther Oncol. 2025 Aug 23;33(4):201040

Inserts

RC2-DelVP1-R585,588A
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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TR-5'UTR-HP-GFP

Plasmid

#80594

Purpose

Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Mutation

Inserted 30 nt hairpin which is targeted by Csy4 …

Promoter

CBA

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2155

Plasmid

#141065

Purpose

AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

Insert

SYN1-EmGFP-WPRE

Use

AAV

Promoter

human Synapsin

Available Since

April 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179429

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_TEV

Plasmid

#58868

Purpose

MESA protease chain with mCherry ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with mCherry ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

Plasmid

#192939

Purpose

FlpO-based digitizer circuit driven by AP1 promoter

Depositor

Wilson Wong

Article

Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

Inserts

AP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2230

Plasmid

#158420

Purpose

AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple253 (PITX3 )

Use

AAV

Promoter

Ple253

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2260

Plasmid

#158423

Purpose

AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

AAV

Promoter

Ple331

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1984

Plasmid

#49115

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1988

Plasmid

#49119

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Expression

Mammalian

Promoter

UGT8

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2058

Plasmid

#49136

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 gfaABC1D-ARL13B-GFP

Plasmid

#253928

Purpose

Expresses ARL13B-GFP in astrocytes (control for ARL13B-BioID2)

Depositor

Baljit Khakh

Insert

ARL13B-GFP (ARL13B Human, Synthetic)

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

gfaABC1D

Available Since

July 21, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

Plasmid

#179840

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC) and eGFP

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212830

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212831

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

Plasmid

#194248

Purpose

Expresses 3 miRNAs targeting mouse alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(mouse) (Snca Mouse)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-flag-mAdrb1-WPRE

Plasmid

#223671

Purpose

AAV expression of flag-mAdrb1 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1 (Adrb1 Mouse)

Use

AAV

Tags

FLAG

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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