We narrowed to 13,668 results for: GAN

1. MTK0_057

   Plasmid

   #123950

   Purpose

   Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI LP on hAAVS1 (Hygro::mRuby)
   

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD280.7

   Plasmid

   #173172

   Purpose

   Expresses SO_4387

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   SO_4387
   

   Expression

   Bacterial

   Promoter

   J23107

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. K401-iLid

   Plasmid

   #122484

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   Kinesin 1-401 iLid His tag
   

   Tags

   His6 and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   T7

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.FRB-3myc-KAP3B

   Plasmid

   #193939

   Purpose

   Mammalian expression of KAP 3B

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   KAP 3B (KIFAP3 Human)
   

   Tags

   FRB

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mKate-P2A-APEX2-NS

   Plasmid

   #67670

   Purpose

   Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-NS
   

   Use

   Gateway multisite middle entry clone

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC22A3

   Plasmid

   #132024

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A3 (SLC22A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

   Plasmid

   #139788

   Purpose

   Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

   Depositor

   Arneet Saltzman

   Article

   Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

   Type

   Empty backbone

   Tags

   6xHis (cleavable by TEV protease) and GST (cleava…

   Expression

   Bacterial

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK0_047

   Plasmid

   #123977

   Purpose

   Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hCLYBL CAS9 Destination - HygroR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PhyB-mCherry-NLS

    Plasmid

    #51571

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    NLS and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF2C-A-RFP

    Plasmid

    #172432

    Purpose

    StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

    Plasmid

    #66781

    Purpose

    Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

    Depositor

    Yishi Jin

    Article

    Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    miniSOG
    

    Tags

    tomm-20 (N terminal 55 aa)

    Expression

    Worm

    Mutation

    G426C

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_A0601

    Plasmid

    #91016

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_D10A nickase
    

    Use

    CRISPR

    Mutation

    D10A

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-17b_Unc104-KIF5B-HALO

    Plasmid

    #169996

    Purpose

    Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

    Insert

    Kinesin-like protein Unc-104 fused with KIF5B
    

    Tags

    HALO-tag, 6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRKB134_GI-DUX4-intron2(-)

    Plasmid

    #83968

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(-) (DUX4 Human)
    

    Expression

    Mammalian

    Promoter

    mouseCMV

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pRKB133_GI-DUX4-intron2(+)

    Plasmid

    #83967

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(+) (DUX4 Human)
    

    Expression

    Mammalian

    Promoter

    mouseCMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pHH0103_S100A11_S-100

    Plasmid

    #109804

    Purpose

    Protein expression and purification of S100A11_S-100

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    S100A11_S-100 (S100A11 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PhyB-mCherry-Htb2

    Plasmid

    #51569

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    HTB2 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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