We narrowed to 908 results for: pgem

1. pCRISPR-HOT-Clover-BlastR

   Plasmid

   #138569

   Purpose

   Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   Clover-BlastR
   

   Use

   CRISPR

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGF130

   Plasmid

   #96971

   Purpose

   S. pombe lem2 knockout cassette with hygro resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lem2 (lem2 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   whole ORF deletion and replaced with hygro resist…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMGF172

   Plasmid

   #96998

   Purpose

   ish1 mCherry insertion cassette with ura4+ gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + ura4(…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gfp-rt-vasa

   Plasmid

   #167322

   Purpose

   Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

   Depositor

   Goro Yoshizaki

   Article

   Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

   Insert

   rt vasa 3'UTRs (ddx4 rainbow trout)
   

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CmCitrine Lck w/ NosUTRs

   Plasmid

   #66785

   Purpose

   expression of fluorescent membranes in sea urchin primordial germ cells

   Depositor

   Amro Hamdoun

   Article

   Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

   Insert

   Lck (LCK Human)
   

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   3' UTR from sea urchin Nanos, 5' UTR fr…

   Mutation

   Tandem repeat of LCK the N-terminal membrane targ…

   Promoter

   T7

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMGF157

   Plasmid

   #96972

   Purpose

   ish1-mCherry insertion cassette with Hygro resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + hygro…

   Promoter

   N/A

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGF170

   Plasmid

   #96997

   Purpose

   ish1-mCherry insertion cassette with NAT resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + NAT r…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMGF169

   Plasmid

   #96996

   Purpose

   ish1 mCherry insertion cassette with G418 resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + G418 …

   Promoter

   N/A

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gfp-zf-vasa

   Plasmid

   #167323

   Purpose

   Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

   Depositor

   Goro Yoshizaki

   Article

   Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

   Insert

   zf vasa 3'UTRs (ddx4 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p189

    Plasmid

    #65207

    Purpose

    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Expression

    Bacterial and Mammalian

    Mutation

    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p221a-GUS

    Plasmid

    #71263

    Purpose

    Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Beta-glucuronidase
    

    Use

    Gateway

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2R3a-TagRFP-OcsT

    Plasmid

    #71270

    Purpose

    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2R3a-Tq2CFP-OcsT

    Plasmid

    #71268

    Purpose

    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2R3a-GUS-OcsT

    Plasmid

    #71267

    Purpose

    Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Beta-glucuronidase
    

    Use

    Gateway

    Tags

    2xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2R3a-3xVenusYFP-OcsT

    Plasmid

    #71271

    Purpose

    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    3 times VenusYFP
    

    Use

    Gateway

    Tags

    octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2R3a-VenusYFP-3AT

    Plasmid

    #71269

    Purpose

    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    4xGly linker and T3A pea Pisum sativum ribulose-1…

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7TS

    Plasmid

    #17091

    Depositor

    Paul Krieg

    Type

    Empty backbone

    Available Since

    June 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. x.Amylase

    Plasmid

    #87132

    Purpose

    To make in situ hybridization probe for Xenopus amylase.

    Depositor

    Francesca Spagnoli

    Insert

    Amylase
    

    Expression

    Mammalian

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only