We narrowed to 14,201 results for: cas9

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pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124540

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Publication

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41, d11-41

Promoter

Minimal ADE

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124538

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Publication

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55G, d11-41

Promoter

Minimal ADE

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Publication

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Expression

Mammalian

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120297

Purpose

AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIA4-2xmiR-122 target sites

Plasmid

#120298

Purpose

AAV vector for expression of AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4-2xmiR-122 binding sites

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONOR-PARK6e5-I368N

Plasmid

#86154

Purpose

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Depositor

Jens Schwamborn

Publication

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

PINK1 exon 5 homology arms (PINK1 Human)

Expression

Bacterial and Mammalian

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Publication

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Publication

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147929)

Plasmid

#80254

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147380)

Plasmid

#80225

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147185)

Plasmid

#80236

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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non-targeting control gRNA (BRDN0001162447)

Plasmid

#80239

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146709)

Plasmid

#80240

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146085)

Plasmid

#80216

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147095)

Plasmid

#80222

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149294)

Plasmid

#80192

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146145)

Plasmid

#80198

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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