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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IGFP

Plasmid

#233005

Purpose

Galactose iduced expression of Gcn4 F124IGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AGFP

Plasmid

#233004

Purpose

Galactose iduced expression of Gcn4 120AGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 K5

Plasmid

#232966

Purpose

Galactose iduced expression of Gcn4 K+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 P+

Plasmid

#232971

Purpose

Galactose iduced expression of Gcn4 Proline in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Proline

Tags

TEV cleavage site

Expression

Yeast

Mutation

S122P, T131F, A141P, E143D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E2K2

Plasmid

#232968

Purpose

Galactose iduced expression of Gcn4 EK+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 EK+

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104K, S117E, S136E, S144K

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3HA

Plasmid

#232996

Purpose

Galactose iduced expression of Gcn4 E+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+

Plasmid

#232984

Purpose

Galactose iduced expression of Gcn4 WW+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F108A+

Plasmid

#232974

Purpose

Galactose iduced expression of Gcn4 F108A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A

Tags

TEV cleavage site

Expression

Yeast

Mutation

F108A, T121L, A141P

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 9acidA

Plasmid

#231867

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

Plasmid

#233038

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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MT2

Plasmid

#223330

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Publication

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)

Tags

3HA-TurboID-EGFP

Expression

Plant

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT3

Plasmid

#223331

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Publication

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)

Tags

3HA-EGFP-miniTurboID

Expression

Plant

Promoter

CaMV35S

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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POC1478

Plasmid

#213804

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1476

Plasmid

#213802

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1477

Plasmid

#213803

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1475

Plasmid

#213801

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeNot1_P

Plasmid

#147239

Purpose

Insect Expression of CeNot1

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeNot1

Expression

Insect

Mutation

one non silent mutation A610T compared to the seq…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6Biso1_1379-1723del1535-1624_P

Plasmid

#147204

Purpose

Bacterial Expression of HsTNRC6Biso1_1379-1723del1535-1624

Depositor

Elisa Izaurralde

Publication

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1379-1723del1535-1624 (TNRC6B Human)

Expression

Bacterial

Mutation

one non silent mutation T1486A compared to the se…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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