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pCP

Plasmid

#204743

Purpose

Cas9-2A-Puro expression vector

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Type

Empty backbone

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5Q-GS3

Plasmid

#204746

Purpose

Expression of Cas9 and human H2AX with the K5Q mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5Q (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5Q mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5R-GS3

Plasmid

#204747

Purpose

Expression of Cas9 and human H2AX with the K5R mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5R (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5R mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134L-GS3

Plasmid

#204750

Purpose

Expression of Cas9 and human H2AX with the K134L mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134L (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134L mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134M-GS3

Plasmid

#204751

Purpose

Expression of Cas9 and human H2AX with the K134M mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134M (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134M mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134A-GS3

Plasmid

#204752

Purpose

Expression of Cas9 and human H2AX with the K134A mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134A mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A-GS3

Plasmid

#204753

Purpose

Expression of Cas9 and human H2A

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A (H2AC20 Human)

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.1-GS3

Plasmid

#204754

Purpose

Expression of Cas9 and human H2A.1

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A.1 (H2AC17 Human)

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.2-GS3

Plasmid

#204755

Purpose

Expression of Cas9 and human H2A.2

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A.2 (H2AC18 Human)

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.L-GS3

Plasmid

#204756

Purpose

Expression of Cas9 and human H2A.L

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AL3 (H2AC6 Human)

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.J-GS3

Plasmid

#204757

Purpose

Expression of Cas9 and human H2A.J

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AJ (H2AJ Human)

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

Plasmid

#216871

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Publication

Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

Insert

Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)

Use

CRISPR

Mutation

mdx mutation in mouse dystrophin

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9ABEmax8.17d

Plasmid

#201955

Purpose

Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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