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pHH0103_LRP5_Ldl-recept-b

Plasmid

#109785

Purpose

Protein expression and purification of LRP5_Ldl-recept-b

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b (LRP5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-198

Plasmid

#31065

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-198 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WKKD

Plasmid

#111905

Purpose

Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

Promoter

Available since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-TGFb1

Plasmid

#101762

Purpose

TGFb1 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

transforming growth factor beta 1 promoter (TGFB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Tags

Expression

Mutation

Promoter

pmSyn1

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-Sox2

Plasmid

#101761

Purpose

Sox2 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SRY-box 2 promoter (SOX2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7-W

Plasmid

#159292

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 (KAT7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10)

Promoter

human EF1a promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p57

Plasmid

#101760

Purpose

p57 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

cyclin dependent kinase inhibitor 1C promoter (CDKN1C Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Ai66(RCRL-tdT) targeting vector

Plasmid

#61578

Purpose

Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-RSR-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

tetO

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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FXYD2-luc

Plasmid

#31056

Depositor

Gerhart Ryffel

Article

Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

Insert

FXYD2 (FXYD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

FXYD2 promoter from -2382 to -1

Promoter

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7-linker-mAID-W

Plasmid

#159294

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 (KAT7 Human)

Use

Lentiviral

Tags

mAID (auxin-inducible degron)-tagged

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10)

Promoter

human EF1a promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2 H358A-pBabe-puro

Plasmid

#22705

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated cDNA: point mutation in codon 358 changi…

Promoter

Available since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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